PPACK Dihydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PPACK Dihydrochloride是其活性成分PPACK（D-苯丙基-L-脯氨酰-L-精氨酸氯甲基酮）的二盐酸盐形式，是一种有效的、选择性的和不可逆的凝血酶抑制剂，抑制人α凝血酶，Ki值为0.24 nM。PPACK与凝血酶活性位点的特异性Ser结合，并与结合位点的His57交联，形成一个四面体的PPACK-凝血酶复合体。该复合体进一步与高亲和性的功能凝血酶受体结合并使其饱和，最终导致凝血酶活化的抑制。PPACK目前正用作抗凝血剂的研究，用于防止血液凝固。研究结果表明，PPACK能够剂量依赖性地抑制凝血酶诱导的血小板聚集。
参考文献：
Jun T. Liu, William Paul, Michael Emerson, Carla Cicala and Clive P. Page. Thrombin inhibitors and anti-coagulants on thrombin-induced embolization in rabbit cranial vasculature. European Journal of Pharmacology 264 (1994) 183-190
Ildiko M. Kovach, Paul Kelley, Carol Eddy, Frank Jordan and Ahmet Baykal. Proton bridging in the interactions of thrombin with small inhibitors. Biochemistry. 2009; 48(30): 7296-7304
Martha E. Lyon, James S. Fine, Paul J. Henderson and Andrew W. Lyon. D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone (PPACK): alternative anticoagulant to heparin salts for blood gas and electrolyte specimens. CLIN. CHEM. 41/7, 1038-1041 (1995)
| Storage | Store at -20°C |
| M.Wt | 523.88 |
| Cas No. | 142036-63-3 |
| Formula | C21H31ClN6O3·2HCl |
| Synonyms | PPACK Dihydrochloride |
| Solubility | ≥49.5 mg/mL in DMSO; ≥32.5 mg/mL in EtOH; ≥37.9 mg/mL in H2O |
| Chemical Name | N-(2-amino-3-phenylpropanoyl)-1-[1-chloro-6-(diaminomethylideneamino)-2-oxohexan-3-yl]pyrrolidine-2-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | C1CC(N(C1)C(CCCN=C(N)N)C(=O)CCl)C(=O)NC(=O)C(CC2=CC=CC=C2)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | PPACK Dihydrochloride是一种有效的、选择性的和不可逆的凝血酶抑制剂，Ki值为0.24 nM。 | |||||
| 靶点 | thrombin | |||||
| IC50 | 0.24 nM (Ki) | |||||
质量控制和MSDS
- 批次:
化学结构
