Poly(I:C)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Poly (I:C), a synthetic double-stranded RNA (dsRNA) analog, is an immunostimulant that acts as the most potent interferon (IFN) inducer. Toll-like receptor- 3 (TLR3) agonist[1]. Its average molecular weight is between 1.5-8kb.
Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the Poly (I:C), yield 3-d mature dendritic cells [2].
Commonly used to stimulate immune system activation in vivo. Also promotes maturation of hPSC-derived cardiomyocytes[3].
References:
[1]. Li Y G, Siripanyaphinyo U, Tumkosit U, et al. Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. Virology Journal, 2012, 9(1): 114.
[2]. Spranger S, Javorovic M, Burdek M, et al. Generation of Th1-Polarizing Dendritic Cells Using the TLR7/8 Agonist CL075. The Journal of Immunology, 2010, 185(1): 738-747.
[3]. Biermann M1, Cai W2, Lang D, et al. Epigenetic Priming of Human Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Accelerates Cardiomyocyte Maturation. Stem Cells, 2019, 37(7): 910-923.
- 1. Zhaoyi Pan, Yujie Feng, et al. "MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules." Virulence. 2022 Dec;13(1):355-369. PMID: 35129074
- 2. Tao Chen, Yu-Xin Lin, et al. "A Low-Producing Haplotype of Interleukin-6 Disrupting CTCF Binding Is Protective against Severe COVID-19." mBio. 2021 Oct 26;12(5):e0137221. PMID: 34634929
- 3. Yueyan Liao, Xiangyu Ke, et al. "The Second-Generation XPO1 Inhibitor Eltanexor Inhibits Human Cytomegalovirus (HCMV) Replication and Promotes Type I Interferon Response." Front Microbiol. 2021 May 3;12:675112. PMID: 34012430
- 4. Lin Lv , Mingzhu Cao, et al. "PRV-encoded UL13 protein kinase acts as an antagonist of innate immunity by targeting IRF3-signaling pathways." Vet Microbiol. 2020 Nov;250:108860. PMID: 33045632
- 5. Song Y, Li P, et al. "CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription." Cell Mol Immunol. 2019 Nov 15. PMID: 31729464
- 6. Qi C, Wang X, et al. "Arginine supplementation improves growth, antioxidant capacity, immunity and disease resistance of juvenile Chinese mitten crab, Eriocheir sinensis." Fish Shellfish Immunol. 2019 Jul 30. pii: S1050-4648(19)30792-2. PMID: 31374316
- 7. Chistyakov DV, Azbukina NV, et al. "Toll-like receptors control p38 and JNK MAPK signaling pathways in rat astrocytes differently, when cultured in normal or high glucose concentrations." Neurochem Int. 2019 Jul 29:104513. PMID: 31369777
| Physical Appearance | A solid (HMW) |
| Storage | Store at -20°C |
| Cas No. | 24939-03-5 |
| Formula | (C10H13N4O8P)x.(C9H14N3O8P)x |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥21.5 mg/mL in H2O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
树突状细胞 |
|
溶解方法 |
可溶于水。若获取更高浓度的溶液，可在37℃下孵育10分钟，随后在超声波浴中摇匀。-20℃以下可储存数月。 |
|
反应条件 |
12.5 mg/ml, 3天 |
|
应用 |
用poly（I：C）处理未成熟DC 72小时诱导产生典型的DC形态改变并显著诱导DC成熟。清除细胞因子后，poly(I:C)处理的DC表型稳定。Poly（I：C）处理的DC胞吞活性下调。 Poly（I：C）处理的DC产生高水平的IL-12和低水平的IL-10。 |
|
注意事项 |
由于实验环境的不同，实际溶解度可能与理论值略有不同，请测试室内所有化合物的溶解度。 |
|
References: [1]. Verdijk R M, Mutis T, Esendam B, et al. Polyriboinosinic polyribocytidylic acid (poly (I: C)) induces stable maturation of functionally active human dendritic cells[J]. The Journal of Immunology, 1999, 163(1): 57-61. |
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化学结构
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