切换导航
关闭
  • 菜单
  • Setting

PMSF

苯甲基磺酰氟
Catalog No.
A2587
丝氨酸蛋白酶不可逆抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 500.00
现货
10g
¥ 454.00
现货
100g
¥ 2,454.00
现货

电话: 021-55669583

邮箱: sales@apexbio.cn

全球经销商

A

背景

PMSF(苯甲基磺酰氟)是丝氨酸蛋白酶的一个不可逆抑制剂,与迟发性神经病的发展有关。它在许多类型的组织中对胞内修复和再生起作用。PMSF是一个长期作用的神经病靶标酯酶(NTE)抑制剂。NTE是一个保护剂,与迟发性多发性神经病(OPIDP)的假定靶点的抑制作用有关。PMSF可以将NTE抑制作用增加90%以上。PMSF还可作为γ-谷酰基转肽酶的一个活性位点定向试剂。

参考文献:
[1].  Thomas Baker, Herbert E. Lowndes, Martin K. Johnson, Irene C. Sandborg. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology. 1980; 46(3-4): 305 – 311.
[2].  Marcello Lotti, Stefano Caroldi, Eugenio Capodicasa, Angelo Moretto. Promotion of organophosphate-induced delayed polyneuropathy by phenylmethanesulfonyl fluoride. Toxicology and Applied Pharmacology. 1991; 108(2): 234 – 241.
[3].  Masayasu Inoue, Seikoh Horiuchi, Yoshimasa Morino. Inactivation of γ-glutamyl transpeptidase by phenylmethanesulfonyl fluoride, a specific inactivator of serine enzymes. Biochemical and Biophysical Research Communications. 1978; 82(4): 1183 – 1188.

文献引用

化学属性

Physical AppearanceA solid
StorageStore at -20°C
M.Wt174.2
Cas No.329-98-6
FormulaC7H7FO2S
Solubilityinsoluble in H2O; ≥17.4 mg/mL in DMSO; ≥28.3 mg/mL in EtOH
Chemical Namephenylmethanesulfonyl fluoride
SDFDownload SDF
Canonical SMILESC1=CC=C(C=C1)CS(=O)(=O)F
运输条件 蓝冰运输或根据您的需求运输。
一般建议不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

试验操作

Cell experiment:[1]

Cell lines

Longitudinal smooth muscle of guinea pig ileum

Reaction Conditions

2 mM PMSF for 30 min incubation

Applications

PMSF inhibited carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15% ~ 19%. The inhibition effect of PMSF on phosphoinositide turnover was due to one or more steps following phosphoinositide breakdown.

Animal experiment:[2]

Animal models

Cats

Dosage form

30 mg/kg

Administered intraperitoneally (i.p.)

Applications

Pretreatment of cats with PMSF (30 mg/kg i.p.) 24 h before the diisopropylfluorophosphate (DFP) injection protected the cats from the delayed neuropathy. No clinical neurotoxic signs were observed at 21 days after DFP exposure. The stimulus-bound repetitive capacity of soleus α-motor nerve terminals was not lost at this time and its incidence was much greater than that which occurred in cats not pretreated with PMSF.

Note

The technical data provided above is for reference only.

References:

1. Sekar MC, Roufogalis BD. Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984, 5(3): 191-203.

2. Baker T, Lowndes HE, Johnson MK, et al. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology, 1980, 46(3-4): 305-311.

质量控制