PluriSIn #1 (NSC 14613)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 14613是多能干细胞的特异性抑制剂（PluriSIn），选择性地去除人多能干细胞（hPSCs），留下大量祖细胞和分化细胞。在15种已确认的PluriSIn中，NSC 14613（亦称PluriSIn＃1）是一种N-酰基苯肼的衍生物，与其它9种PluriSIn共享常见的苯基肼（PH-N[H，C]-NH）结构部分。NSC14613也是硬脂酰辅酶A去饱和酶（SCD1）的抑制剂，抑制hPSCs中SCD1的活性，引起底物和产物失衡，以及随后的棕榈酸酯、硬脂酸酯积累和油酸不足。研究结果表明，NSC14613能诱导hPSCs内质网应激，蛋白质合成衰减和细胞凋亡，可用于防止致瘤未分化细胞形成畸胎瘤。
参考文献：
Ben-David U, Gan QF, Golan-Lev T, Arora P, Yanuka O, Oren YS, Leikin-Frenkel A, Graf M, Garippa R, Boehringer M, Gromo G, Benvenisty N. Selective elimination of human pluripotent stem cells by an oleate synthesis inhibitor discovered in a high-throughput screen. Cell Stem Cell. 2013 Feb 7; 12(2):167-79.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 213.24 |
| Cas No. | 91396-88-2 |
| Formula | C12H11N3O |
| Solubility | insoluble in H2O; ≥15.67 mg/mL in EtOH with gentle warming and ultrasonic; ≥6.75 mg/mL in DMSO |
| Chemical Name | N'-phenylpyridine-4-carbohydrazide |
| SDF | Download SDF |
| Canonical SMILES | C1=CC=C(C=C1)NNC(=O)C2=CC=NC=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | PluriSIn＃1是硬脂酰辅酶A去饱和酶1（SCD1）的抑制剂。 | |||||
| 靶点 | Dehydrogenase | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
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