PKR Inhibitor
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 186-210 nM
PKR Inhibitor is a double-stranded RNA-activated protein kinase (PKR) inhibitor.
Initiation of protein synthesis is reported to be regulated by kinase-dependent phosphorylation reactions. Double-stranded RNA-dependent protein kinase (PKR) is identified as one of these important kinases controlling the initiation step.
In vitro: PKR Inhibitor was identified as a small molecule able to inhibit the autophosphorylation of PKR and to rescue the translation blockade induced by PKR. It was found that PKR Inhibitor prevented not only PKR phosphorylation but also the activation of caspase 3 induced by Ab in SH-SY5Y cells. In addition, the protective effect of PKR Inhibitor on the neuronal cell death induced by endoplasmic reticulum-stress in SH-SY5Y cells has been reported [1].
In vivo: A previous animal study used an in-vivo model of 7-day-old rat exhibiting a high activation of brain PKR to investigate the effects of PKR Inhibitor. Results showed for the first time that acute systemic injection of PKR Inhibitor could specifically inhibit the apoptotic PKR/eIF2a signaling pathway without stimulating the proliferative mTOR/p70S6K signaling mechanism [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Ingrand, S. ,Barrier, L.,Lafay-Chebassier, C., et al. The oxindole/imidazole derivative C16 reduces in vivo brain PKR activation. FEBS Letters 581(23), 4473-4478 (2007).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 268.3 |
Cas No. | 608512-97-6 |
Formula | C13H8N4OS |
Synonyms | C16,GW 506033X,Protein Kinase RNA-activated |
Solubility | ≤2.5mg/ml in DMSO;0.5mg/ml in dimethyl formamide |
Chemical Name | 6,8-dihydro-8-(1H-imidazol-5-ylmethylene)-7H-pyrrolo[2,3-g]benzothiazol-7-one |
SDF | Download SDF |
Canonical SMILES | O=C1NC2=CC=C3C(SC=N3)=C2/C1=C/C4=CNC=N4 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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