PKI 14-22 amide, myristoylated
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Myristoylated PKI (14-22) amide是一种有效的cAMP依赖性蛋白激酶（PKA）抑制剂，阻断脊柱施用8-bromo-cAMP所产生的痛觉过敏[1]。
PKAs是真核生物中cAMP信号的主要介体，在几个生物学过程，如基因表达、细胞凋亡、组织分化和细胞增殖中发挥重要作用。PKAs通过对蛋白底物的丝氨酸/苏氨酸残基进行磷酸化而发挥这些功能[2]。
PKAs在嗜中性粒细胞的吞噬作用中起关键作用。CAMP/PKAs在受体介导的吞噬作用中调节F-肌动蛋白的重组，这是由IgG-FcR的相互作用而触发的。Myristoylated PKI 14-22 amide以剂量依赖的方式减少IgG介导的吞噬反应。在浓度高于10 μM时，PKI 14-22 amide可以抑制中性粒细胞粘附，这使得吞噬的测量难以执行[1]。
由于PKA在哺乳动物细胞中活性的失调与几种类型癌症的发病机理相关，PKI (14–22) amide可用作潜在的疗法，用于治疗这些癌症和许多与PKAs相关的其它疾病[1，2]。
参考文献：
[1] Ydrenius L1, Majeed M, etal. , Activation of cAMP-dependent protein kinase is necessary for actin rearrangements in human neutrophils during phagocytosis. J Leukoc Biol. 2000 Apr;67(4):520-8.
[2] Swierczewski BE, Davies SJ. Developmental regulation of protein kinase A expression and activity in Schistosoma mansoni. Int J Parasitol. 2010 Jul;40(8):929-35.
| Physical Appearance | White lyophilised solid |
| Storage | Store at -20°C |
| M.Wt | 1209.5 |
| Cas No. | 201422-03-9 |
| Formula | C53H100N20O12 |
| Solubility | Soluble to 1 mg/ml in DMSO |
| Chemical Name | (S,Z)-N'1-((S,Z)-1-hydroxy-1-(((2S,3S)-1-hydroxy-1-imino-3-methylpentan-2-yl)imino)propan-2-yl)-2-((Z)-((2S,3Z,5S,6Z,9Z,11S,12Z,14S,15Z,18Z)-2,5,14-tris(3-guanidinopropyl)-1,4,7,10,13,16,19-heptahydroxy-11-((R)-1-hydroxyethyl)-3,6,9,12,15,18-hexaazadotria |
| SDF | Download SDF |
| Canonical SMILES | CCCCCCCCCCCCC/C(O)=N/C/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/C/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](C(O)=N)([H])[C@@](CC)([H])C)([H])C)([H])CC(O)=N)([H])CCCNC(N)=N)([H])CCCNC(N)=N)([H])[C@@](O)([H])C)([H])CCCNC(N)=N |
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