PJ34 hydrochloride
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Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PJ34是一种新型有效的多聚ADP核糖聚合酶（PARP）抑制剂，在无细胞试验中呈剂量依赖性地抑制纯化后的PARP酶，其EC50值为20 nM，PARP参与DNA修复和细胞增殖。不同于其它PARP抑制剂（如3-AB），PJ34不具有任何抗氧化活性，但对PARP的抑制作用比3-AB（EC 50= 200 μM）高10,000倍。对于某些肿瘤类型，PJ34具有神经保护作用并增强化疗效果。研究结果表明，PJ34抑制过氧亚硝酸盐诱导的细胞坏死（EC50 = 20 nM），并呈剂量依赖性地抑制HepG2细胞生长。
参考文献：
Sheng-Hui Huang, Min Xiong, Xiao-Ping Chen, Zhen-Yu Xiao, Yin-Feng Zhao and Zhi-Yong Huang. PJ34, an inhibitor of PARP-1, suppresses cell growth and enhances the suppressive effects of cisplatin in liver cancer cells. Oncology Reports 20: 567-572, 2008
Galaleldin E. Abdelkarim, Karen Gertz, Christoph Harms, Juri Katchanov, Ulrich Dirnagl, Csaba Szabo and Matthias Enders. Protective effects of PJ34, a novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP) in in vitro and in vivo models of stroke. International Journal of Molecular Medicine 7: 255-260, 2001
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 331.8 |
| Cas No. | 344458-15-7 |
| Formula | C17H17N3O2·HCl |
| Synonyms | PJ 34 Hydrochloride |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥16.6 mg/mL in DMSO |
| Chemical Name | 2-(dimethylamino)-N-(6-oxo-5H-phenanthridin-2-yl)acetamide;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CN(C)CC(=O)NC1=CC2=C(C=C1)NC(=O)C3=CC=CC=C32.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
HepG2细胞 |
|
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
|
反应条件 |
0.5、1.0和2.0 mg/L；9天 |
|
实验结果 |
在0.5、1.0和2.0 mg/L的剂量下，PJ34于培养后的第6和9天显著抑制HepG2细胞增殖。 |
| 动物实验 [1]: | |
|
动物模型 |
携带HepG2肿瘤的裸鼠 |
|
给药剂量 |
3 mg/kg；腹腔注射；隔天给药，持续21天 |
|
实验结果 |
在裸鼠中，PJ34抑制HepG2肿瘤生长。 |
|
其它注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。 |
|
References: [1]. Sheng-Hui Huang, Min Xiong, Xiao-Ping Chen, Zhen-Yu Xiao, Yin-Feng Zhao and Zhi-Yong Huang. PJ34, an inhibitor of PARP-1, suppresses cell growth and enhances the suppressive effects of cisplatin in liver cancer cells. Oncology Reports 20: 567-572, 2008 |
|
| PJ34是一种新型有效的PARP-l/2特异性抑制剂，其EC50值为20 nM。. | ||||||
| 靶点 | PARP | |||||
| IC50 | 20 nM (EC50) | |||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
