Phosphatase Inhibitor Cocktail 3 (100X in DMSO)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Yang M, Mariano J, et al. "SARS-CoV-2 papain-like protease (PLpro) plays multiple roles in regulating cellular proteins in the endoplasmic reticulum." Journal of Biological Chemistry, 2023: 105346. PMID: 37838170
- 2. Thuy T P Nguyen, Do-Young Kim, et al. "SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice." Mol Cell. 2021 Sep 16;81(18):3820-3832.e7. PMID: 34233158
- 3. Alpsoy A, Dykhuizen EC. "Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes." J Biol Chem. 2018 Mar 16;293(11):3892-3903. PMID: 29374058
蛋白磷酸化是重要的共价翻译后修饰，可以改变蛋白质的结构构象，并调控靶蛋白的功能，位置和特异性结合。许多细胞过程受蛋白质的可逆磷酸化调控，30％的蛋白质可能在其存在过程中被磷酸化。
由于内源性蛋白的产生和降解处于平衡状态，因此在稳定的环境条件下其细胞水平是稳定的。细胞粗提取物含有许多内源性酶，例如磷酸酶和蛋白酶，其能够降解和改变提取物中的蛋白。提高完整蛋白产量的最佳方法是加入这些酶的抑制剂。
Phosphatase Inhibitor Cocktail 3抑制碱性磷酸酶的L-同功酶和丝氨酸/苏氨酸蛋白磷酸酶，例如蛋白磷酸酶1和2A（PP1和PP2A）。在来自不同动物组织的细胞提取物中对Phosphatase Inhibitor Cocktail 3进行了优化和测试。Phosphatase Inhibitor Cocktail 3可以代替Phosphatase Inhibitor Cocktail 1，两种Cocktail的活性等价。
Phosphatase Inhibitor Cocktail 3含有Cantharidin，Bromotetramisole和Calyculin A。该Phosphatase Inhibitor Cocktail以DMSO的即用溶液提供。
在室温下解冻，然后在实验前以1:100（v / v）稀释至溶液样品（如细胞裂解物或组织提取物）。
应用：WB，Co-IP，pull-down，IF，IHC，kinase assay等。
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| N1686 | Cantharidin | 蛋白磷酸酶1/2A抑制剂 | Natural Products | 56-25-7 | O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O |
| B4750 | (-)-p-Bromotetramisole Oxalate | ALP抑制剂 | Others|Others | 62284-79-1 | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O |
| A4533 | Calyculin A | 蛋白磷酸酶抑制剂 | Chromatin/Epigenetics|Protein Ser/Thr Phosphatases | 101932-71-2 | CC1C(CC2(C(C(C(O2)C(CC(C(C)C(C(C)C=C(C)C(=CC=CC(=CC#N)C)C)O)O)OC)OP(=O)(O)O)(C)C)OC1CC=CC3=COC(=N3)C(C)CCNC(=O)C(C(C(COC)N(C)C)O)O)O |
| -20°C条件下储存，稳定保存12个月以上。当在2-8°C条件下储存时，该产品可稳定保存2个月。 | |||||
