PFI 4
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PFI 4是一种细胞渗透性的\强效的选择性BRPF1 Bromodomain抑制剂,pIC50和pKd值分别为7.1和8.0[1].
BRPF(含bromodomain和PHD finger)家族BRPF1\BRPF2/BRD1和BRP F3作为支架组装MYST家族组蛋白乙酰转移酶(HATs)复合体.BRPF1是一种包含MOZ/MORF转录共激活因子复合体的组分,并将催化HATs与其他亚基ING5和hEAF6.8相连.BRPF1对于维持鱼类骨骼发育和Hox基因表达很重要[1].
在35个bromodomain结合试验的BROMOscan组中,PFI 4表现出优良的BRPF1效力,pKd值为8.0,以及比对其他bromodomains更高的选择性.在细胞蛋白相互作用试验中,PFI 4从Halo标记的组蛋白H3.3中替换出NanoLuc标记的BRPF1 bromodomain,并干扰染色质结合[1].
参考文献：
[1]. Demont EH, Bamborough P, Chung CW, et al. 1,3-Dimethyl Benzimidazolones Are Potent, Selective Inhibitors of the BRPF1 Bromodomain. ACS Med Chem Lett, 2014, 5(11): 1190-1195.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 380.44 |
| Cas No. | 900305-37-5 |
| Formula | C21H24N4O3 |
| Solubility | insoluble in H2O; ≥13.3 mg/mL in DMSO; ≥2.87 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | N-(1,3-dimethyl-2-oxo-6-(pyrrolidin-1-yl)-2,3-dihydro-1H-benzo[d]imidazol-5-yl)-2-methoxybenzamide |
| SDF | Download SDF |
| Canonical SMILES | [H]N(C(C1=CC=CC=C1OC)=O)C2=C(N3CCCC3)C=C(N(C)C(N4C)=O)C4=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
