PFI 3
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PFI 3是有效的polybromo 1和SMARCA4选择性抑制剂,Kd值分别为48和89 nM[1]。
转录激活剂BRG1(SMARCA4)是SWI/SNF家族成员,可调控转录。蛋白polybromo-1(PB1、PBRM1)是BRG1相关因子和肿瘤抑制子。
PFI 3是一种有效的polybromo 1和SMARCA4选择性抑制剂。PFI 3可结合SMARCA2和SMARCA4 bromodomains,Kd值分别为55和110 nM。PFI-3(2 μM)可抑制70%的PBRM1。在基于细胞的染色质结合试验中,PFI-3可以剂量依赖的方式替换GFP标记的SMARCA2,IC50值为5.78 μM。PFI-3是适用于研究SMARCA2/4 bromodomains的细胞通透性探针。在SMARCA4缺陷的A549、H1299和H157细胞系中,PFI-3不具有抗增殖功能,且不能从染色质中替换SMARCA2。在THP-1和MV4-11白血病细胞中,PFI 3不能诱导抗癌表型[1]。
参考文献:
[1]. Vangamudi B, Paul TA, Shah PK, et al. The SMARCA2/4 ATPase domain surpasses the bromodomain as a drug target in SWI/SNF mutant cancers: Insights from cDNA rescue and PFI-3 inhibitor studies. Cancer Res, 2015, pii: canres.3798.2015.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 321.37 |
Cas No. | 1819363-80-8 |
Formula | C19H19N3O2 |
Solubility | insoluble in H2O; ≥15.15 mg/mL in DMSO; ≥2.97 mg/mL in EtOH with ultrasonic |
SDF | Download SDF |
Canonical SMILES | O=C(C1=CC=CC=C1O)/C=C/N(C2)[C@@H]3C[C@H]2N(C4=NC=CC=C4)C3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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