Pergolide mesylate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 410.59 |
| Cas No. | 66104-23-2 |
| Formula | C19H26N2S·CH4O3S |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥6.84 mg/mL in DMSO |
| Chemical Name | (6aR,9R,10aR)-9-(methylsulfanylmethyl)-7-propyl-6,6a,8,9,10,10a-hexahydro-4H-indolo[4,3-fg]quinoline;methanesulfonic acid |
| SDF | Download SDF |
| Canonical SMILES | CCCN1CC(CC2C1CC3=CNC4=CC=CC2=C34)CSC.CS(=O)(=O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
SH-SY5Y神经母细胞瘤细胞 |
|
溶解方法 |
该化合物在DMSO中的溶解度大于10 mM。若获取更高浓度的溶液，可在37℃下孵育10分钟，随后在超声波浴中摇匀。-20℃以下可储存数月。 |
|
反应条件 |
10 nM-50 μM |
|
应用 |
Pergolide以剂量依赖方式保护神经母细胞瘤细胞免受H2O2诱导的神经毒性，其中pergolide的IC50值为约40和60 nM。Pergolide （1 μM）孵育26小时不影响细胞活力。在给予细胞毒素之前，使用pergolide （100 nM）预孵育2小时，不影响多柔比星或顺铂的神经毒性作用。 |
| 动物实验 [2,3]: | |
|
动物模型 |
雌性大鼠 |
|
给药剂量 |
腹腔注射，0.5 mg/kg/day，7天 |
|
应用 |
在切除卵巢的雌性大鼠中，pergolide mesylate显著抑制食物摄入和体重。重复给药Pergolide并逐步减少Pergolide的浓度，抑制食物摄入。Pergolide抑制体重，无药物耐受。Pergolide mesylate（7天，0.5 mg/kg/day, i.p.)治疗的大鼠中，与6-OHDA纹状体输注相关的2,3-DHBA的平均量明显小于对照组。Pergolide治疗导致纹状体组织在体内淬灭羟基自由基形成的能力增强。 |
|
注意事项 |
由于实验环境的不同，实际溶解度可能与理论值略有不同，请测试室内所有化合物的溶解度。 |
|
References: [1]. Uberti D, Piccioni L, Colzi A, et al. Pergolide protects SH-SY5Y cells against neurodegeneration induced by H 2 O 2[J]. European journal of pharmacology, 2002, 434(1): 17-20. [2]. Greene S B, Mathews D, Hollingsworth E M, et al. Behavioral effects of pergolide mesylate on food intake and body weight[J]. Pharmacology Biochemistry and Behavior, 1985, 23(2): 161-167. [3]. Opacka-Juffry J, Wilson A W, Blunt S B. Effects of pergolide treatment on in vivo hydroxyl free radical formation during infusion of 6-hydroxydopamine in rat striatum[J]. Brain research, 1998, 810(1): 27-33. | |
质量控制和MSDS
- 批次:
化学结构
