Pam3CSK4
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pam3CSK4是Toll样受体1/2(TLR1/2)的激动剂[1]。
Toll样受体（TLR）在先天免疫系统中发挥重要作用。TLR主要表达于前哨细胞中，识别由微生物衍生的分子，然后激活免疫细胞应答。
Pam3CSK4是TLR1/2的激动剂。Pam3CSK4以Syk和src依赖性方式增加PLCγ2和接头蛋白LAT的酪氨酸磷酸化。此外，Pam3CSK4诱发血小板活化[1]。
在对粉尘螨过敏原过敏的小鼠鼻炎模型中，Pam3CSK4显著改善鼻过敏症状，并降低支气管肺泡灌洗液（BALF）中总炎症细胞和嗜酸性粒细胞。此外，Pam3CSK4显著增加IFN-γ的释放，减少IL-13和总IgE的水平 [2]。Pam3CSK4诱导巨噬细胞产生NO和TNF-ɑ[3]。在用OVA激发的小鼠哮喘模型中，Pam3CSK4显著降低BALF中嗜酸性粒细胞和总炎症细胞渗透，并抑制OVA诱导的IL-4和IL-5的分泌。此外，Pam3CSK4减少IgE的水平，增加IL-12的生成[4]。
参考文献：
[1]. F?lker K, Klarstr?m-Engstr?m K, Bengtsson T, et al. The Toll-like receptor 2/1 (TLR2/1) complex initiates human platelet activation via the src/Syk/LAT/PLCγ2 signalling cascade. Cell Signal, 2014, 26(2): 279-286.?
[2]. Zhou C, Kang XD, Chen Z. A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen. J Zhejiang Univ Sci B, 2008, 9(4): 279-285.
[3]. Han SH, Kim JH, Seo HS, et al. Lipoteichoic acid-induced nitric oxide production depends on the activation of platelet-activating factor receptor and Jak2. J Immunol, 2006, 176(1): 573-579.
[4]. Patel M, Xu D, Kewin P, et al. TLR2 agonist ameliorates established allergic airway inflammation by promoting Th1 response and not via regulatory T cells. J Immunol, 2005, 174(12): 7558-7563.
- 1. Ma K, Guo J, et al. "Toll-Like Receptor 2-Mediated Autophagy Promotes Microglial Cell Death by Modulating the Microglial M1/M2 Phenotype." Inflammation. 2019 Dec 13. PMID:31834572
- 2. Bi Y, Xu Q, et al. "The combinations-chitosan+Pam(3)CSK(4) and chitosan+MPL: promising immune enhancing adjuvants for anti-caries vaccine PAc." Infect Immun. 2019 Sep 16. pii: IAI.00651-19. PMID:31527122
| Physical Appearance | White lyophilised solid |
| Storage | Store at -20°C |
| M.Wt | 1510.24 |
| Cas No. | 112208-00-1 |
| Formula | C81H156N10O13S |
| Chemical Name | (2S,3Z,5S,6Z,8S,9Z,11S,12Z,14S,15Z,17R)-2,5,8,11-tetrakis(4-aminobutyl)-4,7,10,13,16-pentahydroxy-17-((Z)-(1-hydroxyhexadecylidene)amino)-14-(hydroxymethyl)-24-oxo-21-(palmitoyloxy)-23-oxa-19-thia-3,6,9,12,15-pentaazanonatriaconta-3,6,9,12,15-pentaen-1-oi |
| SDF | Download SDF |
| Canonical SMILES | CCCCCCCCCCCCCCC/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](/C(O)=N/[C@@](C(O)=O)([H])CCCCN)([H])CCCCN)([H])CCCCN)([H])CCCCN)([H])CO)([H])CSCC(OC(CCCCCCCCCCCCCCC)=O)([H])COC(CCCCCCCCCCCCCCC)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
相关生物数据
相关生物数据
