OSI-420 free base
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
OSI-420是OSI-774(erlotinib)的主要代谢产物,而OSI-774(erlotinib)是一种小分子\具有口服活性\有效的EGFR酪氨酸激酶的选择性抑制剂,IC50值为2 nM[1,2].
OSI-420是OSI-774(erlotinib)的活性代谢产物,可竞争结合三磷酸腺苷(ATP)在EGFR胞内结构域的结合位点,选择性地抑制EGFR酪氨酸激酶,防止EGFR自磷酸化.此外,erlotinib可代谢产生ATP,并对EGFR有选择性,通过扰乱线粒体效应,消除线粒体膜电位,释放细胞色素c,从而诱导细胞凋亡[1,2].
参考文献：
[1] Zhang W1, Siu LL, Moore MJ, Chen EX. Simultaneous determination of OSI-774 and its major metabolite OSI-420 in human plasma by using HPLC with UV detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 5;814(1):143-7.
[2] Bonomi P. Erlotinib: a new therapeutic approach for non-small cell lung cancer.Expert Opin Investig Drugs. 2003 Aug;12(8):1395-401.
| Storage | Store at -20°C |
| M.Wt | 379.41 |
| Cas No. | 183321-86-0 |
| Formula | C21H21N3O4 |
| Synonyms | OSI-420, Desmethyl Erlotinib, CP-473420 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥51 mg/mL in DMSO |
| Chemical Name | 2-((4-((3-ethynylphenyl)amino)-7-(2-methoxyethoxy)quinazolin-6-yl)oxy)ethanol |
| SDF | Download SDF |
| Canonical SMILES | C#CC1=CC(NC2=NC=NC3=C2C=C(OCCO)C(OCCOC)=C3)=CC=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | OSI-420是Erlotinib(EGFR抑制剂,IC50值为2 nM)的活性代谢产物. | |||||
| 靶点 | EGFR | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
