ONO-AE3-208
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ONO-AE3-208是EP4拮抗剂[1]。
EP4是一种前列腺素E2受体,与炎性疾病和癌症相关,如前列腺癌。ONO-AE3-208是一种EP4拮抗剂,有望用于治疗前列腺癌。体外实验表明,ONO-AE3-208可以抑制前列腺癌细胞迁移与侵袭。在伤口愈合实验中,10 μM的ONO-AE3-208降低PC3和LNCaP细胞的伤口愈合比例。在Transwell侵袭实验中,0.1 μM的ONO-AE3-208也可以抑制细胞侵袭。据报道,ONO-AE3-208还可以抑制体内骨转移[1]。
E2活化可以增加基质金属蛋白酶(MMP)表达,释放炎性细胞因子,从而促进腹主动脉瘤(AAA)形成,因而ONO-AE3-208也用于研究AAA形成。试验表明,ONO-AE3-208在体内可以呈剂量依赖性地降解弹性纤维并抑制AAA形成[2]。
参考文献:
[1] Song Xu, Zhengyu Zhang, Osamu Ogawa,Takeshi Yoshikawa, Hiromasa Sakamoto, Noboru Shibasaki, akayuki Goto, Liming Wang, Naoki Terada. An EP4 antagonist ONO-AE3-208 suppresses cell invasion, migration and metastasis of prostate cancer. Cell Biochem Biophys. 2014, April.
[2] Utako Yokoyama, Ryo Ishiwata, Mei-Hua Jin, Yuko Kato, Orie Suzuki, Huiling Jin, Yasuhiro Ichikawa, Syun Kumagaya, Yuzo Katayama, Takayuki Fujita, Satoshi Okumura, Motohiko Sato, Yukihiko Sugimoto, Hiroki Aoki, Shinichi Suzuki, Munetaka Masuda, Susumu Minamisawa, Yoshihiro Ishikawa. Inhibition of EP4 Signaling Attenuates Aortic Aneurysm Formation. Plos One. 2012, May. 7, 5, e36724.
2. Jin, Pengfeng, et al. "Lactobacillus fermentum ZYL0401 Attenuates Lipopolysaccharide-Induced Hepatic TNF-α Expression and Liver Injury via an IL-10-and PGE 2-EP4-Dependent Mechanism." (2015): e0126520. PMID:25978374
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 404.43 |
Cas No. | 402473-54-5 |
Formula | C24H21FN2O3 |
Synonyms | AE 3-208;ONO AE3 208 |
Solubility | ≥40.4 mg/mL in DMSO with gentle warming; insoluble in H2O; ≥8.1 mg/mL in EtOH with gentle warming and ultrasonic |
Chemical Name | 4-[4-cyano-2-[2-(4-fluoronaphthalen-1-yl)propanoylamino]phenyl]butanoic acid |
SDF | Download SDF |
Canonical SMILES | CC(C1=CC=C(C2=CC=CC=C21)F)C(=O)NC3=C(C=CC(=C3)C#N)CCCC(=O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | ONO-AE3-208(AE 3-208)是EP4拮抗剂。 | |||||
靶点 | EP4 | |||||
IC50 |
质量控制和MSDS
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