Oligomycin A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Oligomycin A是ATP合成酶的抑制剂,其抑制作用发生于依赖ATP和氧化磷酸化的线粒体耦合膜过程[1]。
Oligomycin A阻断ATP合酶的质子通道,这是ADP通过氧化磷酸化转化为ATP的必要步骤,从而抑制ATP合酶。Oligomycin A对ATP合成的抑制过程可以显著减少电子传递链的电子流动。
来自美国国家癌症研究所的对60种人癌细胞系的研究表明,oligomycin是37000个分子中前0.1%的最具选择性的细胞毒性药物。在HeLa癌细胞系中,H+ -ATP-synthase的抑制剂 oligomycin(5 mg/ml)强烈抑制细胞呼吸,这种抑制作用是与ATP合成相关的。在一系列癌细胞中,100 ng/ml oligomycin在1小时内可完全抑制癌细胞的氧化磷酸化活性,在6小时内可诱导不同程度的糖酵解。作为H + -ATP- synthase F0部分的抑制剂,oligomycin可以抑制TNF诱导的细胞凋亡。使用不同浓度的oligomycin和rotenone减少了高达94%的耗氧量,表明线粒体在这个过程中的重要作用。使用oligomycin可完全消除H2O2的增加[2-4]。
参考文献:?
[1]. Jastroch M, Divakaruni AS, Mookerjee S, et al. Mitochondrial proton and electron leaks. Essays Biochemistry, 2010, 47:53-67.
[2]. Shchepina LA, Pletjushkina OY, Avetisyan AV, et al. Oligomycin, inhibitor of the F-0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. Oncogene, 2002, 53: 8149-8157.
[3]. Salomon AR, Voehringer DW, Herzenberg LA, et al. Understanding and exploiting the mechanistic basis for selectivity of polyketide inhibitors of F0F1-ATPase. Proceedings of The National Academy of Sciences of The United States of America, 2000, 97(26): 14766-14771.
[4]. Alexander R, Adina V, Ivan B, et al. Overcoming intrinsic multi-drug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling JARID1Bhigh? cells. Cancer Cell, 2013, 23(6): 811-825
- 1. Jing Lv, Ying Yi, et al. "Mitochondrial homeostasis regulates definitive endoderm differentiation of human pluripotent stem cells." Cell Death Discov. 2022 Feb 17;8(1):69. PMID: 35177589
- 2. Xingyuan Zhai, Kai Liu, et al. "Mitochondrial C1qbp promotes differentiation of effector CD8+T cells via metabolic-epigenetic reprogramming." Sci Adv. 2021 Dec 3;7(49):eabk0490. PMID: 34860557
- 3. Tian C, Yuan Z, et al. "Inhibition of glycolysis by a novel EGFR/HER2 inhibitor KU004 suppresses the growth of HER2+cancer." Exp Cell Res. 2017 May 19. pii: S0014-4827(17)30297-5. PMID: 28532652
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 791.06 |
Cas No. | 579-13-5 |
Formula | C45H74O11 |
Solubility | insoluble in H2O; ≥17.43 mg/mL in EtOH; ≥9.89 mg/mL in DMSO |
Chemical Name | 4-ethyl-11,12,15,19-tetrahydroxy-6'-(2-hydroxypropyl)-5',10,12,14,16,18,20,26,29-nonamethylspiro[24,28-dioxabicyclo[23.3.1]nonacosa-5,7,21-triene-27,2'-oxane]-13,17,23-trione |
SDF | Download SDF |
Canonical SMILES | CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
人喉癌耐多烯紫杉醇DRHEp2细胞 |
溶解方法 |
在DMSO中的溶解度大于9.9mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
72 h,2 μg/ml |
应用 |
Oligomycin A是F0-ATP酶的抑制剂。Oligomycin A以剂量依赖的方式增加DRHEp2细胞对多烯紫杉醇的敏感性。联合使用Oligomycin A和多烯紫杉醇可增加线粒体ROS的产生。 |
References: [1]. Mizumachi T, Suzuki S, Naito A, et al. Increased mitochondrial DNA induces acquired docetaxel resistance in head and neck cancer cells[J]. Oncogene, 2008, 27(6): 831-838. |