OF-1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
OF-1是一种选择性的BRPF1B和BRPF2 bromodomain抑制剂(Kd值分别为100 nM和500 nM;选择性是多数其他bromodomain的100多倍).
BRPF(BRomodomain and PHD Finger containing)蛋白家族是装备MYST组蛋白乙酰转移酶复合体的支架蛋白.MYST复合体在DNA修复\重组\复制和转录激活中扮演重要角色.
在全长BRPF1B的细胞热变动试验(CETSA)中,OF-1可在1 M下提高热稳定性.在BRPF2 FRAP试验中,OF-1可在5 M下加速FRAP恢复.OF-1有适度的一般毒性.
参考文献：
[1]. OF-1 - A chemical probe for BRPF bromodomains. (2010, October 13). SGC. Retrieved from http://www.thesgc.org/chemical-probes/OF-1
| Storage | Store at -20°C |
| M.Wt | 440.31 |
| Cas No. | 919973-83-4 |
| Formula | C17H18BrN3O4S |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥14.55 mg/mL in DMSO |
| Chemical Name | 4-bromo-N-(6-methoxy-1,3-dimethyl-2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)-2-methylbenzenesulfonamide |
| SDF | Download SDF |
| Canonical SMILES | CN1C2=C(C=C(OC)C(NS(=O)(C3=C(C)C=C(Br)C=C3)=O)=C2)N(C)C1=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
