Nystatin (Fungicidin)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nystatin(Fungicidin)是一种多烯抗真菌抗生素[1].
抗真菌抗生素是一种用于治疗和预防真菌疾病的医药杀菌剂.
Nystatin是一种多烯抗真菌抗生素,对酵母和支原体有效.在液体培养基中,Nystatin在5-20 U/ml浓度下可抑制C. albicans[2].
对包含113株白色念珠菌\54株光滑念珠菌\11株近平滑念珠菌\11株热带念珠菌和11株克柔念珠菌的200个临床分离株中,Nystatin对检测的白色念珠菌分离株和所有非白色念珠菌的MIC90值为4 mg/L.结果表明,白色念珠菌最为易感,而Nystatin可用于治疗非白色念珠菌的念珠菌引起的外阴阴道念珠菌病.对于感染对咪唑基药物具有更高耐受性的非白色念珠菌菌株的女性而言,Nystatin是一种重要的选择[3].
参考文献：
[1]. Childs AJ. Effect of Nystatin on Growth of Candida albicans During Antibiotic Therapy. Br Med J, 1956, 1(4968): 660-662.
[2]. Stewart GT. Laboratory and Clinical Studies with Nystatin in Post-antibiotic Mycotic Infections. Br Med J, 1956, 1(4968): 658-660.
[3]. Choukri F, Benderdouche M, Sednaoui P. In vitro susceptibility profile of 200 recent clinical isolates of Candida spp. to topical antifungal treatments of vulvovaginal candidiasis, the imidazoles and nystatin agents. J Mycol Med, 2014, 24(4): 303-307.
- 1. Wei P, Ning M, et al. "Spiroplasma eriocheiris entered Drosophila Schneider 2 cells and relied on clathrin-mediated endocytosis and macropinocytosis." Infect Immun. 2019 Aug 26. pii: IAI.00233-19. PMID:31451616
- 2. Wang H, Liu W, et al. "Inhibitor analysis revealed that clathrin-mediated endocytosis is involed in cellular entry of type III grass carp reovirus." Virol J. 2018 May 24;15(1):92. PMID:29793525
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 926.09 |
| Cas No. | 1400-61-9 |
| Formula | C47H75NO17 |
| Solubility | ≥30.45 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | (4E,6E,8E,10E,14E,16E,18S,19R,20R,21S,35S)-3-[(2S,3S,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-19,25,27,29,32,33,35,37-octahydroxy-18,20,21-trimethyl-23-oxo-22,39-dioxabicyclo[33.3.1]nonatriaconta-4,6,8,10,14,16-hexaene-38-carboxylic acid |
| SDF | Download SDF |
| Canonical SMILES | CC1C=CC=CCCC=CC=CC=CC=CC(CC2C(C(CC(O2)(CC(C(CCC(CC(CC(CC(=O)OC(C(C1O)C)C)O)O)O)O)O)O)O)C(=O)O)OC3C(C(C(C(O3)C)O)N)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
口腔念珠菌和人颊上皮细胞 |
|
制备方法 |
在DMSO中的溶解度大于30.5 mg/mL。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
|
反应条件 |
1小时 |
|
实验结果 |
在RPMI培养基中，Nystatin对白色念珠菌、热带假丝酵母、克氏梭菌、副伤寒杆菌、光滑假丝酵母菌和吉利蒙念珠菌的最小抑菌浓度分别为0.78 ~ 1.56、1.56 ~ 3.12、3.12、1.56 ~ 3.12、0.78 ~ 1.56和0.39 ~ 0.78 (μg/mL)。与对照组相比，Nystatin显著降低了6种念珠菌对颊上皮细胞的粘附。然而，Nystatin治疗对白色念珠菌分离物的粘附影响最少，其与非白色念珠菌的结果存在显著的差异。 |
| 动物实验 [2]: | |
|
动物模型 |
感染曲霉菌并伴有中性粒细胞减少症状的小鼠 |
|
给药剂量 |
2、4、6或8 mg/kg/day；静脉注射 |
|
实验结果 |
在剂量低至2 mg/kg/day的情况下，与未接受治疗组、盐水组或空脂质体组相比，脂质体Nystatin显著减少曲霉菌诱导的小鼠死亡。在所有处理组中，小鼠在第5天没有显示出曲霉菌感染的迹象。在第52天，8 mg/kg/day脂质体Nystatin治疗组也没有显示出曲霉菌感染的迹象。 |
|
注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。 |
|
References: [1]. Ellepola AN, Panagoda GJ, Samaranayake LP. Adhesion of oral Candida species to human buccal epithelial cells following brief exposure to nystatin. Oral Microbiol Immunol. 1999 Dec;14(6):358-63. [2]. Wallace TL, Paetznick V, Cossum PA, Lopez-Berestein G, Rex JH, Anaissie E. Activity of liposomal nystatin against disseminated Aspergillus fumigatus infection in neutropenic mice. Antimicrob Agents Chemother. 1997 Oct;41(10):2238-43. |
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质量控制和MSDS
- 批次:
化学结构
相关生物数据
