NVP-LCQ195
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NVP-LCQ195是CDKs的pan-抑制剂[1]。
周期蛋白依赖性激酶（CDKs）是一类蛋白激酶，在调节细胞周期中发挥着重要作用。此外，CDKs还参与转录、mRNA加工以及神经细胞分化。
在体外激酶活性试验中，NVP-LCQ195阻断CDK1/cyclin B、CDK2/cyclin A、CDK2/cyclin E和CDK5（包括CDK5 p25和CDK5 p35）的活性，相应IC50值分别为2 nM、2 nM、5 nM和1 nM。此外，NVP-LCQ195也抑制CLK3、CHEK2（CHK2），CDK3/cyclin E、CDK9/cyclin T1、CDK7/cyclin H和CDK6/cyclin D3。在MM.1S细胞中，NVP-LCQ195（2 μM）首先增加处于S和G2/M期的细胞百分比，再增加亚G1期的细胞数目。NVP-LCQ195抑制转录因子表达，包括myc、IRF4和XBP-1[1]。
在接受Bortezomib治疗的多发性骨髓瘤患者中，NVP-LCQ195抑制基因高表达，并显著延长无进展生存期和总生存期[1]。
参考文献：
[1]. McMillin DW, Delmore J, Negri J, et al. Molecular and cellular effects of multi-targeted cyclin-dependent kinase inhibition in myeloma: biological and clinical implications. Br J Haematol, 2011, 152(4): 420-432.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 460.33 |
| Cas No. | 902156-99-4 |
| Formula | C17H19Cl2N5O4S |
| Synonyms | LCQ-195;AT-9311;NVP LCQ195;LCQ 195;AT9311;AT 9311 |
| Solubility | insoluble in H2O; ≥152.4 mg/mL in DMSO; ≥3.28 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 4-[(2,6-dichlorobenzoyl)amino]-N-(1-methylsulfonylpiperidin-4-yl)-1H-pyrazole-5-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | CS(=O)(=O)N1CCC(CC1)NC(=O)C2=C(C=NN2)NC(=O)C3=C(C=CC=C3Cl)Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
