NSC 632839 hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 632839是一种非选择性的异肽酶抑制剂，作用于USP2、USP7和SENP2，IC50值分别为45 μM、37 μM和9.8 μM[1]。
据报道，NSC 632839对纯化的异肽酶具有活性，不仅抑制DUB（USP2、USP7），也抑制deSUMOylase（SENP2）。NSC 632839也抑制转化的成纤维细胞裂解物对Ub模拟物（z-LRGG-AMC）的裂解。然而，NSC 632839对报告酶PLA2没有抑制作用，表明其对异肽酶的抑制是选择性的[1]。NSC 632839阻止从聚泛素化蛋白上去除泛素链，抑制泛素依赖的、蛋白酶体介导的蛋白降解。NSC 632839以凋亡小体不依赖的方式激活细胞凋亡[2，3]。
参考文献：
[1] Nicholson B, Leach CA, Goldenberg SJ, Francis DM, Kodrasov MP, Tian X, Shanks J, Sterner DE, Bernal A, Mattern MR, Wilkinson KD, Butt TR. Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities. Protein Sci. 2008 Jun;17(6):1035-43.
[2] Gupta-Saraf P, Miller CL. HIF-1α downregulation and apoptosis in hypoxic prostate tumor cells infected with oncolytic mammalian orthoreovirus. Oncotarget. 2014 Jan 30;5(2):561-74.
[3] Eldridge AG, O'Brien T. Therapeutic strategies within the ubiquitin proteasome system. Cell Death Differ. 2010 Jan;17(1):4-13.
| Physical Appearance | A solid |
| Storage | Store at 4°C |
| M.Wt | 339.86 |
| Cas No. | 157654-67-6 |
| Formula | C21H21NO·HCl |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥17 mg/mL in DMSO with gentle warming |
| Chemical Name | (3E,5E)-3,5-bis[(4-methylphenyl)methylidene]piperidin-4-one;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CC1=CC=C(C=C1)C=C2CNCC(=CC3=CC=C(C=C3)C)C2=O.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
