NSC 3852
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 3852是组蛋白去乙酰化酶的有效抑制剂[1]。
去乙酰化酶(HADC)可以除去组蛋白中ε-N-乙酰赖氨酸上的乙酰基,使组蛋白更紧密地包裹住DNA,阻止转录。
在MCF-7人乳腺癌细胞中,NSC 3852诱导超氧化物歧化酶催化的DMPO-OH加合物的形成。研究表明,NSC 3852诱导超氧化物和活性氧(ROS)的产生,引发DNA损伤并诱导细胞凋亡。另外,NSC 3852导致G0期细胞累积以及视网膜母细胞瘤蛋白(Rb)的低磷酸化,并减少E2F1和Myc蛋白[1]。在HS68细胞中,NSC 3852抑制弓形虫速殖子传播,EC50值为78.6 nM。在人红血细胞中,NSC 3852抑制恶性疟原虫生长,EC50值为1.3 μM。NSC 3852抑制弓形虫速殖子的传播过程中涉及氧化还原反应的参与[2]。
参考文献:
[1]. Martirosyan A, Leonard S, Shi X, et al. Actions of a histone deacetylase inhibitor NSC3852 (5-nitroso-8-quinolinol) link reactive oxygen species to cell differentiation and apoptosis in MCF-7 human mammary tumor cells. J Pharmacol Exp Ther, 2006, 317(2): 546-552.
[2]. Strobl JS, Seibert CW, Li Y, et al. Inhibition of Toxoplasma gondii and Plasmodium falciparum infections in vitro by NSC3852, a redox active antiproliferative and tumor cell differentiation agent. J Parasitol, 2009, 95(1): 215-223.
Physical Appearance | A solid |
Storage | Store at RT |
M.Wt | 174.16 |
Cas No. | 3565-26-2 |
Formula | C9H6N2O2 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥7.35 mg/mL in DMSO |
Chemical Name | 5-nitrosoquinolin-8-ol |
SDF | Download SDF |
Canonical SMILES | C1=CC2=C(C=CC(=C2N=C1)O)N=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次: