NSC 319726
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 319726是突变型p53的活性剂,IC50值为8 nM。
NSC 319726属于胺苯硫脲家族。在老鼠成纤维细胞系Balb/c 3T3中,NSC 319726对突变型p53(相比于对照野生型p53)细胞生长表现出更高的抑制性。NSC 319726对WI38人成纤维细胞(p53 WT)是没有作用的。在三个不同的卵巢癌细胞系TOV112D(p53R175H)、OVCAR3(p53R248W)和SKOV3(p53-/-)中,NSC 319726处理导致p53R175突变体中产生细胞凋亡。NSC 319726通过诱导突变型p53的构象朝“WT-like”结构改变而恢复p53功能,这个“WT-like”结构可以被WT的特异性抗体识别。在TOV112D (p53R175H)移植瘤细胞中也发现了NSC 319726(1 mg/kg)对细胞生长的抑制。此外,锌离子螯合和ROS变化对NSC319726诱导的细胞凋亡有很大的影响[1]。
参考文献:
[1] Yu X, Vazquez A, Levine AJ, Carpizo DR. Allele-specific p53 mutant reactivation. Cancer Cell. 2012 May 15;21(5):614-25.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 234.32 |
Cas No. | 71555-25-4 |
Formula | C11H14N4S |
Solubility | insoluble in H2O; ≥3.43 mg/mL in EtOH with gentle warming and ultrasonic; ≥8.1 mg/mL in DMSO |
Chemical Name | N-[(Z)-1-pyridin-2-ylethylideneamino]azetidine-1-carbothioamide |
SDF | Download SDF |
Canonical SMILES | CC(=NNC(=S)N1CCC1)C2=CC=CC=N2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
描述 | NSC 319726是突变型p53的活性剂,对于成纤维细胞表达突变型p53R175的IC50值为8 nM。 | |||||
靶点 | mutant p53 | |||||
IC50 | 8 nM (in fibroblasts expressing the p53R175 mutation) |
质量控制和MSDS
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