NHS-SS-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NHS-SS-biotin [Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate]是一个长链裂解的胺反应性生物素化试剂，由于结构中横桥的存在，它具有24.3 A长度的间隔臂。长间隔臂增加了共价修饰分子与双环生物素环之间的长度，从而增强分子与亲和素或链亲和素探针的结合能力。NHS-SS-biotin的NHS酯基与蛋白和其它分子的胺基反应形成酰胺键，并释放sulfo-NHS。NHS-SS-Biotin是水不溶性的分子，在加入反应性水溶液之前需要先溶于有机溶剂中。
参考文献：
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
| Storage | Store at -20°C该产品在溶液中不稳定，建议现配现用 |
| M.Wt | 504.64 |
| Cas No. | 122266-55-1 |
| Formula | C19H28N4O6S3 |
| Synonyms | NHS-SS-Biotin,Biotin disulfide N-hydroxysuccinimide ester |
| Solubility | ≥28.79 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | 2,5-dioxopyrrolidin-1-yl 3-((2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethyl)disulfanyl)propanoate |
| SDF | Download SDF |
| Canonical SMILES | O=C1CCC(N1OC(CCSSCCNC(CCCC[C@@H]2SC[C@@](N3)([H])[C@]2([H])NC3=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Biotinylation method [1]: | |
|
Sample |
hippocampal neurons |
|
Preparation method |
Soluble in DMSO or DMF. |
|
Reaction Conditions |
1.5 mg/ml, 4 ℃ for 1 h |
|
Applications |
Neurons were washed with the artificial cerebrospinal fluid（ACSF） at 37 ℃, and incubated with 1.5 ml of 1.5 mg/ml NHS-SS-biotin with gentle shaking at 4 ℃ for 1 h. After washing, neurons were switched to the neuronal culture medium and incubated at 37 ℃. At indicated times of incubation, neurons were cooled to 4 ℃ and un-endocytosed surface biotin was cleaved by incubating in the glutathione cleavage buffer (50 mM glutathione, 75 mM NaCl, 10 mM EDTA, 1% BSA, and 0.075 N NaOH). Neurons were lysed in the modified RIPA buffer (50 mM Tris–HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxylate, 1 mM EDTA, and protease inhibitors). Lysates were cleared by centrifugation at 10,000g for 10 min at 4 ℃ and incubated at 4 ℃ over-night with 70 μl of 50% streptavidin beads. Endocytosis of ErbB proteins was assayed using cleavable biotin. Bead-associated proteins were subjected to Western blot analysis. |
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References: [1]. Yu Liu, Yan-Mei Tao, Ran-Sook Woo, Wen-Cheng Xiong, Lin Mei. Stimulated ErbB4 internalization is necessary for neuregulin signaling in neurons. Biochemical and Biophysical Research Communications 354 (2007) 505–510. |
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| 描述 | NHS-SS-Biotin是一种细胞通透性的和伯胺反应性的生物素化试剂。 | |||||
| 靶点 | proteins | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
