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NHS-LC-Biotin

现货
Catalog No.
A8004
生物素化试剂,长链。
组合的产品项目
规格价格库存 数量
100mg
¥ 600.00
现货
1g
¥ 4,300.00
现货

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Background

NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end. The NHS ester group at the end of NHS-LC-biotin covalently binds to amine groups in proteins and other molecules forming a stable amide linkage and releasing the NHS group. The 6-aminocaproic acid spacer of NHS-LC-biotin greatly increases the length between a covalently modified molecule and the bicyclic biotin rings leading to a better binding potential for avidin or streptavidin probes. NHS-LC-biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

文献引用

1. Zhang X, Qiu M, et al. "Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation." Pharmaceutics. 2018 Dec 18;10(4). pii: E286. PMID:30567356
2. Zhu J, Lee KY, et al. "Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors." PLoS Genet. 2017 Feb 17;13(2):e1006634. PMID:28212375

Features

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm is 22.4 angstroms; it consists of the biotin valeric acid group extended by a 6-atom chain

Chemical Properties

StorageStore at -20°C
M.Wt454.54
Cas No.72040-63-2
FormulaC20H30N4O6S
SynonymsSuccinimidyl 6-(biotinamido)hexanoate
Solubility≥22.727mg/mL in DMSO, <2.22 mg/mL in EtOH, <2.43 mg/mL in H2O
Chemical Name(2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate
SDFDownload SDF
Canonical SMILESC1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

生物素化方法[1]:

样品

金黄色葡萄球菌

溶解方法

可以溶于DMSO或DMF。

反应条件

60 mg/ml, 室温1 h

应用

固定好的金黄色葡萄球菌细胞悬液用pH 为8.3,浓度为0.2 M 的NaHCO3溶液稀释至2%。用DMSO将NHS-LC-biotin配成浓度为60 mg/ml的溶液,随后将细胞悬液加入其中。室温下,将两者轻轻的上下颠倒混匀60分钟后用PD-1O凝胶过滤柱去除多余的生物素将反应停止,随后再用大量的浓度为0.01 mM并且含0.15M NaCl 的Tris缓冲液清洗. 生物素标记的蛋白可以用碱性磷酸酶偶联的链霉亲和素(AP-STRAV)与其反应进行检测。AP-STRAV的结合可以说明对硝基苯磷酸(pNPP)的水解功能。

References:

[1]. TRUC NGUYEN, BERHANE GHEBREHIWET, AND ELLINOR I. B. PEERSCHKE. Staphylococcus aureus Protein A Recognizes Platelet gC1qR/p33: a Novel Mechanism for Staphylococcal Interactions with Platelets. INFECTION AND IMMUNITY 2000, p. 2061–2068.

生物活性

描述 NHS-LC-Biotin是一种胺反应性的生物素化试剂。
靶点            
IC50            

质量控制

化学结构

NHS-LC-Biotin

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NHS-LC-Biotin

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NHS-LC-Biotin

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NHS-LC-Biotin

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NHS-LC-Biotin