NHS-LC-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NHS-LC-biotin(succinimidyl-6-(biotinamindo)hexanoate),也被称为NHS-X-biotin,是D-生物素的衍生物,一种胺反应性的生物素化试剂,由D-生物素的戊酸侧链通过间隔臂与NHS酯基团连接而形成。NHS-LC-biotin末端的NHS酯基与蛋白或其它分子上的胺基共价结合形成稳定的酰胺键,并释放NHS基团。NHS-LC-biotin的6-氨基己酸间隔臂极大地增加了共价修饰分子与双环生物素环之间的长度,导致对亲和素或链霉素探针更好的结合潜力。NHS-LC-biotin在水溶液中是不溶的,在加入缓冲反应之前需先溶解于有机溶剂中。
参考文献:
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- 1. Cong H, Wang K, et al. "Tuning the Brightness and Photostability of Organic Dots for Multivalent Targeted Cancer Imaging and Surgery." ACS Nano. 2020;14(5):5887-5900. PMID:32356972
- 2. JIUHE ZHU. "The regulation of neuronal excitability by epilepsy-associated gene Nedd4-2." University of Illinois. Urbana-Champaign, 2019
- 3. Kamperman T, Koerselman M, et al. "Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs." Nat Commun. 2019 Sep 25;10(1):4347. PMID:31554812
- 4. Zhang X, Qiu M, et al. "Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation." Pharmaceutics. 2018 Dec 18;10(4). pii: E286. PMID:30567356
- 5. Zhu J, Lee KY, et al. "Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors." PLoS Genet. 2017 Feb 17;13(2):e1006634. PMID:28212375
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm is 22.4 angstroms; it consists of the biotin valeric acid group extended by a 6-atom chain
Storage | Store at -20°C |
M.Wt | 454.54 |
Cas No. | 72040-63-2 |
Formula | C20H30N4O6S |
Synonyms | Succinimidyl 6-(biotinamido)hexanoate |
Solubility | ≥22.73 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
Chemical Name | (2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate |
SDF | Download SDF |
Canonical SMILES | C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
生物素化方法[1]: | |
样品 |
金黄色葡萄球菌 |
溶解方法 |
可以溶于DMSO或DMF。 |
反应条件 |
60 mg/ml, 室温1 h |
应用 |
固定好的金黄色葡萄球菌细胞悬液用pH 为8.3,浓度为0.2 M 的NaHCO3溶液稀释至2%。用DMSO将NHS-LC-biotin配成浓度为60 mg/ml的溶液,随后将细胞悬液加入其中。室温下,将两者轻轻的上下颠倒混匀60分钟后用PD-1O凝胶过滤柱去除多余的生物素将反应停止,随后再用大量的浓度为0.01 mM并且含0.15M NaCl 的Tris缓冲液清洗. 生物素标记的蛋白可以用碱性磷酸酶偶联的链霉亲和素(AP-STRAV)与其反应进行检测。AP-STRAV的结合可以说明对硝基苯磷酸(pNPP)的水解功能。 |
References: [1]. TRUC NGUYEN, BERHANE GHEBREHIWET, AND ELLINOR I. B. PEERSCHKE. Staphylococcus aureus Protein A Recognizes Platelet gC1qR/p33: a Novel Mechanism for Staphylococcal Interactions with Platelets. INFECTION AND IMMUNITY 2000, p. 2061–2068. |
描述 | NHS-LC-Biotin是一种胺反应性的生物素化试剂。 | |||||
靶点 | ||||||
IC50 |
质量控制和MSDS
- 批次: