NHS-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NHS-Biotin (C14H18O5N3S)是一个N-羟基琥珀酰亚胺生物素,是三个类似的EZ-Link NHS-Biotin试剂中最短的一个,它能够简单、有效地将溶液中的抗体、蛋白和任何其它含有伯胺的生物分子进行生物素化。NHS-Biotin为研究人员提供了标记和检测的可能性,其中生物素结合的空间位阻是重要影响因素。由于不带电且含有简单的烷基链间隔臂,NHS-Biotin是膜通透性的,可用于细胞内标记。
NHS-Biotin是最常用的生物素化试剂。NHS活化的生物素可以有效地与碱性缓冲液中的伯胺基(-NH2)反应形成稳定的酰胺键。蛋白或抗体通常都含有几个伯胺,可作为标记的靶标,包括赖氨酸残基的侧链以及每个多肽的N末端。
参考文献:
Fouassier, L. , Yun, C. C., Fitz, J. G. and Doctor, R. B. (2000). Evidence for Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) Self-association through PDZ-PDZ Interactions. J. Biol. Chem. 275, 25039-25045.
Nunomura, W. , Takakuwa, Y., Parra, M., Conboy, J.G., & Mohandas, N. (2000) Regulation of protein 4.1R, p55 and glycophorin C ternary complex in human erythrocyte membrane. J. Biol. Chem. 275(32): 24540-24546.
Chiu, N. H. & T.K. Christopoulos. 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin. Chem. 45: 1954–1959..
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• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 341.38 |
Cas No. | 35013-72-0 |
Formula | C14H19N3SO5 |
Synonyms | Biotin N-hydroxysuccinimide ester, Biotin-OSU, Biotin NHS, Biotin SE |
Solubility | insoluble in EtOH; insoluble in H2O; ≥17.07 mg/mL in DMSO |
Chemical Name | (2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate |
SDF | Download SDF |
Canonical SMILES | C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
生物素化方法[1]: | |
样品 |
红细胞 |
溶解方法 |
可以溶于DMSO或DMF。 |
反应条件 |
30分钟 |
应用 |
首先,用DMSO将NHS-biotin溶解成100 mg/mL,然后用90%的DMSO溶液将其稀释到20 mg/mL。用0.2 μm孔径的注射过滤器对Biotin溶液进行过滤消毒后,再用6倍体积的0.9%生理盐水将Biotin溶液再次稀释。在加NHS-biotin溶液标记血液前,先将血液平衡到室温。Biotin-DMSO生理盐水溶液注入到血袋中并搅拌混匀30 min,可以得到生物素浓度为0.04 pg的红细胞biotin。 |
References: [1]. J.E. Tomlinson, E. Taberner, R.C. Boston, S.D. Owens, and R.D. Nolen-Walston. Survival Time of Cross-Match Incompatible Red Blood Cells in Adult Horses. J Vet Intern Med 2015;29:1683–1688 |
描述 | NHS-Biotin是一种最简单的NHS-酯活化的生物素化试剂,可用于细胞内标记。 | |||||
靶点 | intracellular proteins | |||||
IC50 |
质量控制和MSDS
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