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NHS-Biotin

现货
Catalog No.
A8002
生物素化试剂
组合的产品项目
规格价格库存 数量
100mg
¥ 370.00
现货
1g
¥ 3,000.00
现货

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Background

NHS-Biotin (C14H18O5N3S) is N-hydroxysuccinimido biotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling.

NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.

 Ref:

  1. Fouassier, L., Yun, C. C., Fitz, J. G. and Doctor, R. B. (2000). Evidence for Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) Self-association through PDZ-PDZ Interactions. J. Biol. Chem. 275, 25039-25045.
  2. Nunomura, W., Takakuwa, Y., Parra, M., Conboy, J.G., & Mohandas, N. (2000) Regulation of protein 4.1R, p55 and glycophorin C ternary complex in human erythrocyte membrane. J. Biol. Chem. 275(32): 24540-24546.
  3. Chiu, N.H. & T.K. Christopoulos. 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin. Chem. 45: 1954–1959..

文献引用

Features

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
M.Wt341.38
Cas No.35013-72-0
FormulaC14H19N3SO5
SynonymsBiotin N-hydroxysuccinimide ester, Biotin-OSU, Biotin NHS, Biotin SE
Solubility≥17.069mg/mL in DMSO, <2.63 mg/mL in EtOH, <2.67 mg/mL in H2O
Chemical Name(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate
SDFDownload SDF
Canonical SMILESC1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

生物素化方法[1]:

样品

红细胞

溶解方法

可以溶于DMSO或DMF。

反应条件

30 分钟

应用

首先,用DMSO将NHS-biotin溶解成100 mg/mL,然后用90%的DMSO溶液将其稀释到20 mg/mL。用0.2-μm孔径的注射过滤器对Biotin溶液进行过滤消毒后,再用6倍体积的0.9%生理盐水将Biotin溶液再次稀释。在加NHS-biotin溶液标记血液前,先将血液平衡到室温。Biotin-DMSO-生理盐水溶液注入到血袋中并搅拌混匀30 min,可以得到生物素浓度为0.04 pg的红细胞-biotin。

References:

[1]. J.E. Tomlinson, E. Taberner, R.C. Boston, S.D. Owens, and R.D. Nolen-Walston. Survival Time of Cross-Match Incompatible Red Blood Cells in Adult Horses. J Vet Intern Med 2015;29:1683–1688

生物活性

描述 NHS-Biotin是一种最简单的NHS-酯活化的生物素化试剂,可用于细胞内标记。
靶点 intracellular proteins          
IC50            

质量控制

化学结构

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