Necrostatin 2
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Necrostatin 2 (Nec-2),Necrostatin 1 (Nec-1)的类似物,是一种新型的和有效的细胞坏死抑制剂,其EC50为50 nM。
细胞坏死是一种坏死性细胞死亡机制。在防止细胞凋亡的条件下,凋亡刺激通过死亡结构域受体与其相应配体结合以诱导细胞坏死。
Nec-2对缺血性脑中风动物模型有效。许多坏死抑制剂衍生物被研发用于实验。关于Nec-2的报道有限,但与其它Necrostatin相关的报道也存在。在体外,Nec-1,坏死的小分子抑制剂,也是死亡结构域受体相关调节激酶RIP1的选择性变构抑制剂。
参考文献:
[1]. Teng X, Keys H, Jeevanandam A, Structure-activity relationship study of [1,2,3]thiadiazole necroptosis inhibitors. Bioorg Med Chem Lett. 2007 Dec 15;17(24):6836-40.
- 1.Sieber J, Wieder N, et al. "GDC-0879, a BRAF(V600E) Inhibitor, Protects Kidney Podocytes from Death." Cell Chem Biol. 2017 Dec 6. PMID:29249695
- 2.Ma Y, Walsh MJ, et al. "CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors." Cell Host Microbe. 2017 May 10;21(5):580-591.e7. PMID:28494239
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 277.71 |
Cas No. | 852391-19-6 |
Formula | C13H12ClN3O2 |
Synonyms | MTH-DL-Tryptophan, Necroptotic Inhibitor |
Solubility | Soluble in DMSO |
Chemical Name | (5R)-5-[(7-chloro-1H-indol-3-yl)methyl]-3-methylimidazolidine-2,4-dione |
SDF | Download SDF |
Canonical SMILES | CN1C(=O)C(NC1=O)CC2=CNC3=C2C=CC=C3Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | Necrostatin 2 (Nec-2)是RIPK2激酶活性的小分子抑制剂。 | |||||
靶点 | RIPK2? | |||||
IC50 |