MPP dihydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Qing Wang, Fengqi Huang, et al. "The ERβ‐CXCL19/CXCR4‐NFκB pathway is critical in mediating the E2-induced inflammation response in the orange-spotted grouper (Epinephelus coioides)." J Steroid Biochem Mol Biol. 2021 Sep;212:105926. PMID: 34091027
- 2. Xiaoyu Hang, Zhenyu Zhang, et al. "Estrogen Protects Articular Cartilage by Downregulating ASIC1a in Rheumatoid Arthritis." J Inflamm Res. 2021 Mar 12;14:843-858. PMID: 33737825
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 542.5 |
| Cas No. | 911295-24-4 |
| Formula | C29H31N3O3·2HCl |
| Solubility | <54.25mg/ml in DMSO |
| Chemical Name | 4-(1-(4-hydroxyphenyl)-4-methyl-5-(4-(2-(piperidin-1-yl)ethoxy)phenyl)-1H-pyrazol-3(2H)-ylidene)cyclohexa-2,5-dienone dihydrochloride |
| SDF | Download SDF |
| Canonical SMILES | OC1=CC=C(C=C1)N(C(C(C=C2)=CC=C2OCCN3CCCCC3)=C/4C)NC4=C(C=C5)\C=CC5=O.Cl.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
原代海马细胞 |
|
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
|
反应条件 |
1 pM ~ 1 μM |
|
实验结果 |
在原代海马细胞中，单独使用E4或与MPP Dihydrochloride联合使用显著降低H2O2诱导的LDH活性。此外，E4，E4 + MPP Dihydrochloride或E4 + MPP Dihydrochloride + PHTTP显著增加细胞存活率。上述结果表明单独的ERα抑制或ERα和ERβ抑制均不影响E4依赖性细胞存活。然而，E4 ﹢ PHTTP（单独抑制ERβ）显著下调细胞存活率。 |
|
References: [1]. Tskitishvili E, Pequeux C, Munaut C, Viellevoye R, Nisolle M, Nol A, Foidart JM. Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study. J Endocrinol. 2017 Jan;232(1):85-95. | |
质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
相关生物数据
