ML161
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ML161是PAR1的选择性抑制剂，IC50值为0.26 μM。
蛋白酶激活受体-1（PAR-1）是特异的G蛋白偶联受体，属于蛋白酶活化受体家族，其活化诱导了一系列下游信号事件，导致多种与肿瘤进展相关的细胞应答[1] 。
ML161是一种强效的PAR1抑制剂，与已知的ALK抑制剂crizotinib具有不同的选择性。在人血小板中，通过检测P-选择素表达发现，ML161以剂量依赖性方式抑制凝血酶诱导的血小板的活化[1]。在颗粒分泌中，ML161以剂量依赖的方式抑制P-选择素的表达，EC50值为0.3 μM，也抑制SFLLRN诱导的血栓形成[2]。
参考文献：
[1]. Dockendorff, C., et al., Discovery of 1,3-Diaminobenzenes as Selective Inhibitors of Platelet Activation at the PAR1 Receptor. ACS Med Chem Lett, 2012. 3(3): p. 232-237.
[2]. VerPlank, L., et al., Chemical Genetic Analysis of Platelet Granule Secretion-Probe 3, in Probe Reports from the NIH Molecular Libraries Program. 2010, National Center for Biotechnology Information (US): Bethesda (MD).
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 361.23 |
| Cas No. | 423735-93-7 |
| Formula | C17H17BrN2O2 |
| Solubility | insoluble in H2O; ≥16.4 mg/mL in DMSO; ≥46.9 mg/mL in EtOH with ultrasonic |
| Chemical Name | 2-bromo-N-[3-(butanoylamino)phenyl]benzamide |
| SDF | Download SDF |
| Canonical SMILES | CCCC(=O)NC1=CC=CC(=C1)NC(=O)C2=CC=CC=C2Br |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | ML-161是PAR1的变构抑制剂，IC50值为0.26 μM。 | |||||
| 靶点 | PAR1 | |||||
| IC50 | 0.26 μM | |||||
质量控制和MSDS
- 批次:
化学结构
