Methylprednisolone
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Methylprednisolone是一种合成的糖皮质激素受体激动剂,用于迅速抑制炎症。
体外实验:低剂量methylprednisolone(2-10 mg/kg)显著抑制TNF生成,而高剂量Methylprednisolone(50 mg/kg)增加LPS诱导的IL-10水平。0.01到100 μg/ ml Methylprednisolone也增加LPS激活小鼠腹腔巨噬细胞中IL-10的生物合成[1]。在WG患者和对照中,methylprednisolone (MP)下调自发的和葡萄球菌肠毒素B(SEB)诱导的外周血单核细胞(PBMC)释放趋化因子[2]。0.25 mM methylprednisolone直接抑制皮肤培养中的棘层松解[3]。
在体实验:在SCI后立即给予30 mg/kg(i.v.)methylprednisolone降低55%的TNF-α表达(P < 0.01)和NF-kB结合活性。Methylprednisolone可以抑制由TNFα-NF-kB级联诱导的创伤后炎症活动[4]。静脉内注射MP(30 mg/kg)使ED1阳性细胞的数量在发梢残端中减少82%,在尾端残端中减少66%。在成年大鼠中,在脊髓横断后不久迅速施用MP导致ED1阳性细胞长期减少和脊髓组织损失,减少前庭绒毛膜纤维的残余,并且减少损伤后1和2周时病变附近的前庭神经纤维的短暂发芽。损伤后2、4和8周,MP显著减少两个残端的组织损失[5]。
临床试验:在国家急性脊髓损伤研究(NASCIS)中心诊断的受伤8小时内的急性脊髓损伤患者中,MP治疗48小时,在损伤后6周(P = 0.09)和6个月(P = 0.07)运动得到改善。在急性脊髓损伤患者中,以每小时每千克5.4 mg输注23小时后,30 mg/kg MP改善神经系统恢复。在MP(30 mg/kg)治疗的患者中,14天时的死亡率显著增加二次感染[7]。在严重的肾性血管炎和狼疮肾炎患者中,MP已经进入临床试验。
参考文献:
Marchant A, Amraoui Z, Gueydan C, et al. Methylprednisolone differentially regulates IL‐10 and tumour necrosis factor (TNF) production during murine endotoxaemia[J]. Clinical & Experimental Immunology, 1996, 106(1): 91-96.
Torheim E A, Yndestad A, Bjerkeli V, et al. Increased expression of chemokines in patients with Wegener's granulomatosis modulating effects of methylprednisolone in vitro[J]. Clinical & Experimental Immunology, 2005, 140(2): 376-383.
Swanson D L, Dahl M V. Methylprednisolone inhibits pemphigus acantholysis in skin cultures[J]. Journal of investigative dermatology, 1983, 81(3): 258-260.
Xu J, Fan G, Chen S, et al. Methylprednisolone inhibition of TNF-α expression and NF-kB activation after spinal cord injury in rats[J]. Molecular brain research, 1998, 59(2): 135-142.
Oudega M, Vargas C G, Weber A B, et al. Long‐term effects of methylprednisolone following transection of adult rat spinal cord[J]. European Journal of Neuroscience, 1999, 11(7): 2453-2464.
Bracken M B, Shepard M J, Holford T R, et al. Administration of methylprednisolone for 24 or 48 hours or tirilazad mesylate for 48 hours in the treatment of acute spinal cord injury: results of the Third National Acute Spinal Cord Injury Randomized Controlled Trial[J]. Jama, 1997, 277(20): 1597-1604.
Bracken M B, Shepard M J, Collins W F, et al. A randomized, controlled trial of methylprednisolone or naloxone in the treatment of acute spinal-cord injury: results of the Second National Acute Spinal Cord Injury Study[J]. New England Journal of Medicine, 1990, 322(20): 1405-1411.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 374.48 |
Cas No. | 83-43-2 |
Formula | C22H30O5 |
Solubility | insoluble in H2O; ≥15.35 mg/mL in DMSO; ≥9.5 mg/mL in EtOH with ultrasonic |
Chemical Name | (6S,8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-6,10,13-trimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-one |
SDF | Download SDF |
Canonical SMILES | CC1CC2C3CCC(C3(CC(C2C4(C1=CC(=O)C=C4)C)O)C)(C(=O)CO)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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