Mebendazole
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Mebendazole is a broad-spectrum microtubule-disrupting anthelmintic that is active against adult or larval cestodes, and human intestinal nematodes [1][2]. Mebendazole had good potency against Giardia isolates with IC50 for a 24-h was 0.19 μM and for a 4-h exposure was 2.7 μM, respectively. And inhibition of Giardia growth in vitro was maintained beyond 72 to 96 h [2].
Mebendazole is a broad-spectrum anthelmintic and a tubulin-disrupting agent. Mebendazole inhibited melanoma growth with an average IC50 of 0.32 μmol/L and induced apoptosis through the intrinsic and extrinsic mitochondrial pathways. In melan-a, M-14, and SK-Mel-19 cells, Mebendazole caused overall microtubular network disarray. In M-14 and SK-Mel-19 melanoma cells, Mebendazole led to Bcl-2 phosphorylation, which then promoted melanoma apoptosis [3]. In human lung cancer cell lines, Mebendazole arrested cells at the G2-M phase before the onset of apoptosis [4]. Also, Mebendazole potently inhibited Hh signaling and slowed the growth of Hh-driven human medulloblastoma cells [6]. Additionally, mebendazole stabilized the transcriptional activator HIF-1α and its downstream targets, preventing oxidative neuronal death in primary neurons [7].
In nu/nu mice bearing human tumor xenografts, Mebendazole inhibited tumor growth and significantly reduced the number and size of tumors [4][5].
References:
[1]. Seo BS, Cho SY, Kang SY, et al. Anthelmintic Efficacy Of Methyl-5-Benzoylbenzimidazole-2-Carbamate(Mebendazole) Against Multiple Helminthic Infections. Kisaengchunghak Chapchi. 1977 Jun;15(1):11-16.
[2]. Morgan UM, Reynoldson JA, Thompson RC. Activities of several benzimidazoles and tubulin inhibitors against Giardia spp. in vitro. Antimicrob Agents Chemother. 1993 Feb;37(2):328-31.
[3]. Doudican N, Rodriguez A, Osman I, et al. Mebendazole induces apoptosis via Bcl-2 inactivation in chemoresistant melanoma cells. Mol Cancer Res. 2008 Aug;6(8):1308-15.
[4]. Mukhopadhyay T, Sasaki J, Ramesh R, et al. Mebendazole elicits a potent antitumor effect on human cancer cell lines both in vitro and in vivo. Clin Cancer Res. 2002 Sep;8(9):2963-9.
[5]. Sasaki J, Ramesh R, Chada S, et al. The anthelmintic drug mebendazole induces mitotic arrest and apoptosis by depolymerizing tubulin in non-small cell lung cancer cells. Mol Cancer Ther. 2002 Nov;1(13):1201-9.
[6]. Larsen AR, Bai RY, Chung JH, et al. Repurposing the antihelmintic mebendazole as a hedgehog inhibitor. Mol Cancer Ther. 2015 Jan;14(1):3-13.
[7]. Aleyasin H, Karuppagounder SS, Kumar A, et al. Antihelminthic benzimidazoles are novel HIF activators that prevent oxidative neuronal death via binding to tubulin. Antioxid Redox Signal. 2015 Jan 10;22(2):121-34.
Storage | Store at -20°C |
M.Wt | 295.3 |
Cas No. | 31431-39-7 |
Formula | C16H13N3O3 |
Synonyms | NSC 184849,R 17635 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥13 mg/mL in DMSO |
Chemical Name | N-(6-benzoyl-1H-benzimidazol-2-yl)-carbamic acid, methyl ester |
SDF | Download SDF |
Canonical SMILES | O=C(C1=CC=C(NC(NC(OC)=O)=N2)C2=C1)C3=CC=CC=C3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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