MAP kinase fragment [Multiple species]
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
丝裂原活化蛋白激酶(MAP激酶)片段的氨基酸序列为Lys-Tyr-Ile-His-Ser-Ala-Asn-Val-Leu。
MAP激酶是丝氨酸/苏氨酸特异性蛋白激酶,属于CMGC(CDK / MAPK / GSK3 / CLK)激酶家族。
MAPK途径是由多种胞外信号激活的蛋白信号级联反应,包括生长因子、细胞因子和环境应激刺激。通过活化特定的转录因子和其它调节蛋白,激活的MAPK途径调节许多基本细胞功能,包括分化、增殖和凋亡。
MAPKs参与各种刺激诱导的细胞应答,这些刺激包括促细胞分裂剂、渗透胁迫、热休克和促炎细胞因子。MAPK蛋白反复涉及癌症和自身免疫疾病的发病机制,使它们成为药物开发的靶标。
Figure 1: x-ray of an ERK2 MAP kinase in its active form.
参考文献:
1. Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S (December 2002). "The protein kinase complement of the human genome". Science 298 (5600): 1912–34.
2. Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, Berman K, Cobb MH (April 2001). "Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions". Endocr. Rev. 22 (2): 153–83.
3. Bandyopadhyay S. et al. “A human MAP kinase interactome”. Nature Methods. 7(2010);801-805.
4. Chang, L. & Karin, M. Mammalian MAP kinase signalling cascades. Nature 410, 37–40 (2001).
5. Widmann, C., Gibson, S., Jarpe, M.B. & Johnson, G.L. Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol. Rev. 79, 143–180 (1999).
6. Kolch, W., Calder, M. & Gilbert, D. When kinases meet mathematics: the systems biology of MAPK signalling. FEBS Lett. 579, 1891–1895 (2005).
7. Johnson, G.L. & Lapadat, R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298, 1911–1912 (2002).
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1044.2 |
Formula | C48H77N13O13 |
Synonyms | H2N-Lys-Tyr-Ile-His-Ser-Ala-Asn-Val-Leu-OH |
Solubility | ≥104.4 mg/mL in DMSO; ≥20.83 mg/mL in H2O; ≥49.2 mg/mL in EtOH |
SDF | Download SDF |
Canonical SMILES | NC(CCCCN)C(NC(CC1=CC=C(O)C=C1)C(NC(C(CC)C)C(NC(CC2=CN=CN2)C(NC(CO)C(NC(C)C(NC(CC(N)=O)C(NC(C(C)C)C(NC(CC(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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