LY2334737
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
LY2334737是一种临床有效的抗肿瘤剂gemcitabine的口服前药[1]。
LY2334737具有羧酸酯酶2（CES2）的水解活性。米氏动力学参数为：Km值为43 μM，Vmax值为40 nmol/min/mg蛋白。此外，LY2334737在NCl-60组中具有细胞毒性，对单独的PC-GFP、HCT-116模拟转染子和HCT-116 CES2转染子细胞系的EC50值分别为0.512±0.016 μM、0.402±0.018 μM和0.034±0.002 μM。除此之外，亲代SK-OV-3细胞对LY2334737毒性的敏感性比SK-OV-3敲除株高约4倍，EC50值分别为1.1 μM和4.5 μM[1]。
参考文献：
[1] Pratt SE1, Durland-Busbice S, Shepard RL, Heinz-Taheny K, Iversen PW, Dantzig AH. Human carboxylesterase-2 hydrolyzes the prodrug of gemcitabine (LY2334737) and confers prodrug sensitivity to cancer cells. Clin Cancer Res. 2013 Mar 1;19(5):1159-68.
| Storage | Store at -20°C |
| M.Wt | 389.39 |
| Cas No. | 892128-60-8 |
| Formula | C17H25F2N3O5 |
| Solubility | Soluble in DMSO |
| Chemical Name | N-[1-[(2R,4R,5R)-3,3-difluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-2-oxopyrimidin-4-yl]-2-propylpentanamide |
| SDF | Download SDF |
| Canonical SMILES | CCCC(CCC)C(=O)NC1=NC(=O)N(C=C1)C2C(C(C(O2)CO)O)(F)F |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
