Latrunculin A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Latrunculin A is a bioactive 2-thiazolidinone macrolide derived from the red sea sponge Latrunculia magnifica, that sequesters G-actin and prevents F-actin assembly. It binds monomeric actin with 1:1 stoichiometry and can be used to block actin polymerization both in vitro and cells[1,2].
Latrunculin A (1-10 μM) causes tumor cytoskeleton disaggregation in ten minutes[3]. Treatment of cells with latrunculin A at 10 μM overnight strongly inhibits actin synthesis [4].
References：
[1]. Yarmola E G, Somasundaram T, Boring T A, et al. Actin-latrunculin A structure and function. The Journal of Biological Chemisty, 2000, 275(36): 28120-28127.
[2]. Loubéry S, Wilhelm C, Hurbain I, et al. Different microtubule motors move early and late endocytic compartments. Traffic, 2008, 9(4): 492-509.
[3]. Hayot C, Debeir O, Van Ham P, et al. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicology and Applied Pharmacology, 2006, 211(1): 30-40.
[4]. Lyubimova A, Bershadsky A D, Ben-Ze'ev A. Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. Journal of Cellular Biochemistry, 1999, 76(1): 1-12.
| Physical Appearance | A solution in ethanol |
| Storage | Store at -20°C |
| M.Wt | 421.55 |
| Cas No. | 76343-93-6 |
| Formula | C22H31NO5S |
| Solubility | Limited solubility, soluble in DMSO |
| Chemical Name | (R)-4-((1R,4Z,8E,10Z,12S,15R,17R)-17-hydroxy-5,12-dimethyl-3-oxo-2,16-dioxabicyclo[13.3.1]nonadeca-4,8,10-trien-17-yl)thiazolidin-2-one |
| SDF | Download SDF |
| Canonical SMILES | O[C@@](C1)([C@H]2NC(SC2)=O)O[C@H](CC[C@@H](/C=C\C=C\CC/C(C)=C\3)C)C[C@H]1OC3=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
SV-80 cells |
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Reaction Conditions |
10 μM latrunculin A |
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Applications |
Latrunculin A treatment (2 h) induced a retraction of the cell body, leaving behind long cellular processes, while stress fibers were not apparent in such cells. Analysis of the distribution of actin between a Triton X-100-soluble and an insoluble fraction showed that while in control cells about 50% of actin was in the Triton X-100-soluble fraction, in latrunculin A-treated cells (12 h) nearly all actin became Triton X-100 soluble. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Lyubimova A, Bershadsky AD, Ben-Ze'ev A. Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. Journal of Cellular Biochemistry, 1999, 76(1): 1-12. |
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质量控制和MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
相关生物数据
