L-Acetylcarnitine (hydrochloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
L-Acetylcarnitine, also named as ALCAR, an acetylated form of L-carnitine, fosters the uptake of acetyl coenzyme A (CoA) into mitochondria during fatty acid oxidation, raises the production of acetylcholine, and triggers the synthesis of membrane phospholipid and protein. ALCAR has been explored for use as an acetylcholine mimic as well as in targeting cardiovascular pathologies. Acetyl-CoA is the primary substrate for the tricarboxylic acid cycle in mitochondria and modulates the reserves of free CoA which is essential to the function of the tricarboxylic acid cycle.
In vitro: The expression of tumor antigen CA-125 in ovarian cancer cells was not affected by ALCAR. Comparing to the control, the growth of SKOV-3 cells was remarkably decreased when incubated with ALCAR. The proliferation of SKOV-3 cells was decreased slightly but significantly when cells were incubated at higher ALCAR concentrations. In addition, ALCAR had no effect on the expression of the nerve growth factor receptors on SKOV-3 or OVCAR-3 ovarian cancer cells [1].
In vivo: Male Flinders Sensitive Line (FSL) rats and male CD1 mice, exposed to model genetic and environmentally induced depression, respectively, were injected intraperitoneally with ALCAR 100 mg/kg for 21 days. ALCAR, as a long-lasting and rapid antidepressant, functioned via the epigenetic regulation of type 2 metabotropic glutamate (mGlu2) receptors in FSL rats and in mice. Additionally, ALCAR raised the transcription of Grm2 gene encoding for the mGlu2 receptor via increasing the levels of acetylated H3K27 bound to the Grm2 promoter and gaining the acetylation of NF-KB-p65 subunit. [2].
References:
[1]. Engle, D., Belisle, J., Gubbels, J., Petrie, S., Hutson, P., Kushner, D., & Patankar, M. Effect of acetyl-l-carnitine on ovarian cancer cells' proliferation, nerve growth factor receptor (Trk-A and p75) expression, and the cytotoxic potential of paclitaxel and carboplatin. Gynecologic Oncology. 2009; 112(3): 631-636.
[2]. Nasca, C., Xenos, D., Barone, Y., Caruso, A., Scaccianoce, S., & Matrisciano, F. et al. L-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors. Proceedings of The National Academy of Sciences. 2013; 110(12): 4804-4809.
Storage | Store at -20°C |
M.Wt | 239.70 |
Cas No. | 5080-50-2 |
Formula | C9H18ClNO4 |
Synonyms | Acetyl-L-Carnitine,ALCAR |
Solubility | ≥11 mg/mL in DMSO with gentle warming; ≥18.23 mg/mL in EtOH; ≥58.5 mg/mL in H2O |
Chemical Name | (R)-2-acetoxy-3-carboxy-N,N,N-trimethylpropan-1-aminium chloride |
SDF | Download SDF |
Canonical SMILES | O=C(O)C[C@@H](OC(C)=O)C[N+](C)(C)C.[Cl-] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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