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KN-93 hydrochloride

现货
Catalog No.
B1306
CaMK II抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 1,300.00
现货
1mg
¥ 450.00
现货
5mg
¥ 1,160.00
现货
10mg
¥ 1,440.00
现货
25mg
¥ 3,180.00
现货

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Background

KN-93 hydrochloride is a potent inhibitor of Ca2+/calmodulin-dependent protein kinase II (CaMK II) with Ki value of 0.37μM [1].

KN-93 is an inhibitor of CaMK II. It exerts its effect by competing for the calmodulin binding site of CaMK II with IC50 value of 370nM. In isolated gastric parietal cells, KN-93 shows a potent inhibition of acid secretion induced by carbachol and histamine with IC50 values of 0.15μM and 0.3μM, respectively. It also inhibits forskolin induced aminopyrine uptake with IC50 value of 0.6μM. KN-93 is also found to have effects on the H+, K+-ATPase. 10μM KN-93 and 20μM KN-93 can inhibit 26% and 35% activity of the enzyme, respectively. Moreover, KN-93 is reported to act as an extracellular blocker of Kv channels through enhancing C-type inactivation [1, 2].

References:
[1] Mamiya N, Goldenring J R, Tsunoda Y, et al. Inhibition of Acid Secretion in Gastric Parietal Cells by the Ca2+/Calmodulin-Dependent Protein Kinase II Inhibitor KN-93. Biochemical and biophysical research communications, 1993, 195(2): 608-615.
[2] Rezazadeh S, Claydon T W, Fedida D. KN-93 (2-[N-(2-hydroxyethyl)]-N-(4-methoxybenzenesulfonyl)] amino-N-(4-chlorocinnamyl)-N-methylbenzylamine), a calcium/calmodulin-dependent protein kinase II inhibitor, is a direct extracellular blocker of voltage-gated potassium channels. Journal of Pharmacology and Experimental Therapeutics, 2006, 317(1): 292-299.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt537.5
Cas No.1956426-56-4
FormulaC26H30Cl2N2O4S
Solubility≥26.9mg/mL in DMSO
Chemical Name(E)-N-(2-(((3-(4-chlorophenyl)allyl)(methyl)amino)methyl)phenyl)-N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide hydrochloride
SDFDownload SDF
Canonical SMILESCN(CC1=CC=CC=C1N(S(C2=CC=C(OC)C=C2)(=O)=O)CCO)C/C([H])=C([H])/C3=CC=C(Cl)C=C3.Cl
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验[1]:

细胞系

NIH 3T3成纤维细胞

溶解方法

在DMSO中的溶解度大于10 mM。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。

反应条件

24-72 h,24 μM

应用

KN-93是钙离子/钙调蛋白依赖性蛋白激酶II(CaMK-II)的抑制剂。它以剂量依赖的方式抑制成纤维细胞CaMK-II的活性及细胞生长,将细胞可逆地阻滞在G1期,诱导细胞凋亡。

动物实验[2]:

动物模型

8-24周龄的CaMKIV转基因小鼠

剂量

10-30 μmol/kg,腹腔注射

应用

和野生型小鼠比起来,在CaMKIV转基因小鼠里KN-93能够显著抑制异丙肾上腺素诱导的心律失常,表明在心肌肥厚的小鼠模型里CaMKII是一个心律失常分子。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。

References:

[1]. Tombes R M, Grant S, Westin E H, et al. G1 cell cycle arrest and apoptosis are induced in NIH 3T3 cells by KN-93, an inhibitor of CaMK-II (the multifunctional Ca2+/CaM kinase)[J]. Cell growth & differentiation: the molecular biology journal of the American Association for Cancer Research, 1995, 6(9): 1063.

[2]. Wu Y, Temple J, Zhang R, et al. Calmodulin kinase II and arrhythmias in a mouse model of cardiac hypertrophy[J]. Circulation, 2002, 106(10): 1288-1293.

质量控制

化学结构

KN-93 hydrochloride