Kartogenin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Kartogenin是促进多能间充质干细胞（MSCs）分化为软骨细胞的强效诱导剂，EC50值为100 nM [1]。
MSCs是一种干细胞，能够分化成不同的细胞类型，包括软骨细胞、成骨细胞、脂肪细胞和肌细胞。
Kartogenin（KGN）以剂量依赖的方式有效诱导hMSCs分化成软骨细胞 [1]。在小鼠前骨骼间充质细胞中，KGN刺激软骨结节和滑膜关节的形成，增强软骨伸长率和刺激趾间内陷、肌腱成熟和域间压实。KGN增加TGFβ1的表达，而TGFβ1刺激软骨结节形成。此外，KGN增加Smad蛋白的磷酸化，Smad介导BMP和TGFβ信号 [2]。
在急性手术模型和慢性关节损伤模型中，kartogenin具有软骨保护作用。 Kartogenin打乱细丝蛋白A和转录因子核心结合因子β亚基（CBFβ）的相互作用，调节CBFβ-RUNX1转录，从而诱导软骨[1]。在BALB/C小鼠的真皮中，KGN诱导I型胶原蛋白的合成，活化TGF-β的Smad4/smad5信号传导途径[3]。
参考文献：
[1]. Johnson K, Zhu S, Tremblay MS, et al. A stem cell-based approach to cartilage repair. Science, 2012, 336(6082): 717-721.?
[2]. Decker RS, Koyama E, Enomoto-Iwamoto M, et al. Mouse limb skeletal growth and synovial joint development are coordinately enhanced by Kartogenin. Dev Biol, 2014, 395(2): 255-267.
[3]. Wang J, Zhou J, Zhang N, et al. A heterocyclic molecule kartogenin induces collagen synthesis of human dermal fibroblasts by activating the smad4/smad5 pathway. Biochem Biophys Res Commun, 2014, 450(1): 568-574.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 317.34 |
| Cas No. | 4727-31-5 |
| Formula | C20H15NO3 |
| Solubility | insoluble in H2O; ≥10.6 mg/mL in DMSO; ≥2.22 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 2-[(4-phenylphenyl)carbamoyl]benzoic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(C1=CC=CC=C1C(O)=O)NC2=CC=C(C=C2)C3=CC=CC=C3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
