K02288
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
K02288是ALK2的高选择性抑制剂，IC50值为1.1 nM [1]。
K02288是ALK2的2-氨基吡啶类抑制剂。对于同源激酶ALK1-6和ActRIIA具有高选择性。K02288对BMP受体ALK1和 ALK2显示出抑制效力，IC50值范围为1-2 nM，对ALK3和ALK6的IC50值为5-34 nM，对ALK4的IC50值为302 nM，对ALK5的IC50值为321 nM。K02288具有广泛的激酶选择性。在200种人类激酶中，0.1 μM的K02288仅对ABL和ARG具有超过50％的抑制，1 μM浓度只进一步抑制另外6种激酶[1]。
K02288也影响ALK2相关的BMP信号。在C2C12细胞中，使用 BMP4或BMP6作为配体时，K02288剂量依赖性地减少Smad的磷酸化。此外，K02288诱导Tg（BRE：MRFP）转基因斑马鱼胚胎的背部化表型[1]。
参考文献：
[1] Sanvitale CE, Kerr G, Chaikuad A, Ramel MC, Mohedas AH, Reichert S, Wang Y, Triffitt JT, Cuny GD, Yu PB, Hill CS, Bullock AN. A new class of small molecule inhibitor of BMP signaling. PLoS One. 2013 Apr 30;8(4):e62721.?
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 352.38 |
| Cas No. | 1431985-92-0 |
| Formula | C20H20N2O4 |
| Solubility | insoluble in H2O; ≥17.6 mg/mL in DMSO; ≥2.5 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 3-[6-amino-5-(3,4,5-trimethoxyphenyl)pyridin-3-yl]phenol |
| SDF | Download SDF |
| Canonical SMILES | COC1=CC(=CC(=C1OC)OC)C2=C(N=CC(=C2)C3=CC(=CC=C3)O)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | K02288是I型BMP受体的选择性抑制剂，对ALK2、ALK1和ALK6的IC50值分别为1.1、1.8和6.4 nM。 | |||||
| 靶点 | ALK2 | ALK1 | ALK6 | ALK3 | ALK4 | ALK5 |
| IC50 | 1.1 nM | 1.8 nM | 6.4 nM | 34.4 nM | 302 nM | 321 nM |
质量控制和MSDS
- 批次:
化学结构
