Isotretinoin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Storage | Store at -20°C |
| M.Wt | 300.44 |
| Cas No. | 4759-48-2 |
| Formula | C20H28O2 |
| Solubility | insoluble in H2O; ≥13.8 mg/mL in DMSO; ≥24.3 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | (2Z,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenoic acid |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Primary human sebocytes |
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Reaction Conditions |
7 ~ 14 d incubation |
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Applications |
Isotretinoin decreased sebocyte proliferation in a dose- and time-dependent manner, with IC50 values being 10 μM after a 7-d incubation and 1 μM after a 14-d incubation, respectively. Isotretinoin (8 d) also inhibited the synthesis of triglycerides, wax/stearyl esters and free fatty acids, and modulated keratin expression in primary human sebocytes at a concentration of 0.1 μM. |
| Animal experiment:[2] | |
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Animal models |
Male inbred Lewis (LEW, RT11) and Fisher (F344, RT11v1) rats, 200 ~ 220 g |
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Dosage form |
2 mg/kg/day Administered orally for 8 weeks |
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Applications |
Isotretinoin reduced chronic rejection damage and decreased mRNA expression of IFN-γ and IL-10 in allografts in chronic Fisher344➛Lewis transplant mice, an allograft nephropathy model. Thus, isotretinoin could serve as a potent immunosuppressive and anti-fibrotic agent able to prevent and inhibit progression of chronic allograft nephropathy. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Zouboulis CC, Korge B, Akamatsu H, et al. Effects of 13-cis-retinoic acid, all-trans-retinoic acid, and acitretin on the proliferation, lipid synthesis and keratin expression of cultured human sebocytes in vitro. Journal of Investigative Dermatology, 1991, 96(5): 792-797. 2. Adams J, Kiss E, Arroyo AB, et al. 13-cis retinoic acid inhibits development and progression of chronic allograft nephropathy. The American Journal of Pathology, 2005, 167(1): 285-298. |
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质量控制和MSDS
- 批次:
化学结构
