ISCK03
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ISCK03 is an inhibitor of SCF-mediated c-kit activation.
The signaling downstream of SCF/c-kit plays a key role in the development of various mammalian cells, such as mast cells, melanocytes, primordial germ cells, and hematopoietic progenitor cells.
In vitro: Previous study showed that the pretreatment of 501mel cells with ISCK03 could dose-dependently inhibit SCF-induced c-kit phosphorylation. ISCK03 could also inhibit the phosphorylation of p44/42 ERK mitogen-activated protein kinase that was known to be involved in SCF/c-kit downstream signaling. However, ISCK03 was not able to inhibit hepatocyte growth factor-induced phosphorylation of p44/42 ERK proteins [1].
In vivo: To determine the in vivo efficacy, ISCK03was orally administered to depilated C57BL/6 mice. Results showed that the oral administration of ISCK03 could induce the dose-dependent depigmentation of newly regrown hair, and this was reversed with cessation of ISCK03 treatment. In addition, the topical application of ISCK03 could promote the depigmentation of UV-induced hyperpigmented spots on n brownish guinea pig skin. Moreover, the Fontana–Masson staining analyses showed epidermal melanin was diminished in spots treated with ISCK03 [1].
Clinical trial: Up to now, ISCK03 is still in the preclinical development stage.
References:
[1] Y. J. Na, H. S. Baek, S. M. Ahn, et al. [4-t-Butylphenyl]-N-(4-imidazol-1-yl phenyl)sulfonamide (ISCK03) inhibits SCF/c-kit signaling in 501mel human melanoma cells and abolishes melanin production in mice and brownish guinea pigs. Biochemical Pharmacology 74(5), 780-786 (2007).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 355.5 |
Cas No. | 945526-43-2 |
Formula | C19H21N3O2S |
Synonyms | c-Kit Inhibitor II,Stem-Cell Factor/c-Kit Inhibitor |
Solubility | ≤2mg/ml in ethanol;25mg/ml in DMSO;30mg/ml in dimethyl formamide |
Chemical Name | 4-(1,1-dimethylethyl)-N-[4-(1H-imidazol-1-yl)phenyl]-benzenesulfonamide |
SDF | Download SDF |
Canonical SMILES | O=S(NC1=CC=C(N2C=NC=C2)C=C1)(C3=CC=C(C(C)(C)C)C=C3)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
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