IOX 1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IOX 1(5-羧基-8HQ)是组蛋白去甲基化酶JMJD的抑制剂,对JMJD2E的IC50值为0.2 μM[1].
Jumonji结构域(JMJD)是一种组蛋白去甲基化酶,充当转录抑制蛋白.
IOX 1是组蛋白去甲基化酶JMJD的抑制剂.在FDH偶联实验中,IOX 1抑制JMJD2E的IC50值为0.2 μM.在MALDI-TOF MS实验中,IOX 1抑制JMJD2E\JMJD2A\FISH和PHD2的IC50值分别为2.4\1.7\20.5和14.3 μM.在HeLa细胞中,IOX 1增加H3K9me3的荧光强度,这表明IOX 1抑制JMJD2A诱导的H3K9me3去甲基化,IC50值为86.5 μM.另外,IOX 1具有一定的细胞毒性,IC50值为291.6 μM[1] .
参考文献:
[1]. King ON, Li XS, Sakurai M, et al. Quantitative high-throughput screening identifies 8-hydroxyquinolines as cell-active histone demethylase inhibitors. PLoS One, 2010, 5(11): e15535.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 189.17 |
Cas No. | 5852-78-8 |
Formula | C10H7NO3 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥5.75 mg/mL in DMSO |
Chemical Name | 8-hydroxyquinoline-5-carboxylic acid |
SDF | Download SDF |
Canonical SMILES | OC1=C2N=CC=CC2=C(C(O)=O)C=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | IOX1 is a potent and broad-spectrum inhibitor of 2OG oxygenases. | |||||
Targets | 2OG oxygenases | |||||
IC50 |