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Influenza Hemagglutinin (HA) Peptide

现货
Catalog No.
A6004
HA标签肽
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 2,000.00
现货
5mg
¥ 1,240.00
现货
25mg
¥ 4,940.00
现货

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Background

HA tag,Complexes containing plasmid DNA, transferrin-polylysine conjugates, and polylysine-conjugated peptides derived from the N-terminal sequence of the influenza virus hemagglutinin subunit HA-2 have been used for the transfer of luciferase or -galactosidase marker genes to K562 cells, HeLa cells, and BNL CL.2 hepatocytes. The presence of these influenza peptide conjugates in the DNA complexes renders the complexes active in membrane disruption in a liposome leakage assay and results in a substantial augmentation of the transferrin-polylysine-mediated gene transfer.

Fusogenic peptides derived from the N-terminal sequence of the influenza virus hemagglutinin subunit HA-2 is part of the DNA complexes and the resulting augmentation of gene transfer to cultured cells1.

In the influenza virus, the hemagglutinin (HA) protein mediates both binding of the virus to the cell surface and the subsequent fusion of viral and cellular membranes. HA is composed of a receptor-binding subunit, denoted HA1, and a fusogenic subunit, denoted HA2. The native HA1yHA2 complex, as found on the surface of the native virus, is fusioninactive. For influenza virus, membrane fusion is regulated by the conformational state of the hemagglutinin (HA) protein, which switches from a native (nonfusogenic) structure to a fusion-active (fusogenic) conformation when exposed to the acidic environment of the cellular endosome. The native structure of HA is trapped in a metastable state and that the fusogenic conformation is released by destabilization of native structure2.

References:
1. E. WAGNER, C. PLANK et al, Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle. Proc. Nati. Acad. Sci. USA Vol. 89, pp. 7934-7938, September 1992
2. C. M. CARR, C. CHAUDHRY, P. S. KIM et al. Influenza hemagglutinin is spring-loaded by a metastable native conformation. Proc. Natl. Acad. Sci. USA Vol. 94, pp. 14306–14313

文献引用

1. Xiong X, Liu X, et al. "Ribosomal protein S27-like regulates autophagy via the β-TrCP-DEPTOR-mTORC1 axis." Cell Death Dis. 2018 Nov 13;9(11):1131. PMID:30425236
2. Xu X, Xu J, et al. "Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response." Cell. 2018 Sep 27. pii: S0092-8674(18)31183-8. PMID:30318148
3. Lampert F, Stafa D, et al. "The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation." Elife. 2018 Jun 18;7. pii: e35528. PMID:29911972

Chemical Properties

StorageDesiccate at -20°C
M.Wt1102.15
Cas No.92000-76-5
FormulaC53H67N9O17
SynonymsH-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-OH
Solubility≥55.1075mg/mL in DMSO, ≥100.4 mg/mL in EtOH, ≥46.2 mg/mL in H2O
Chemical NameInfluenza Hemagglutinin (HA) Peptide
SDFDownload SDF
Canonical SMILESCC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC(CC2=CC=C(C=C2)O)C(=O)NC(C)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C4CCCN4C(=O)C(CC5=CC=C(C=C5)O)N
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

生物活性

描述 Influenza Hemagglutinin (HA) Peptide是从流感病毒血凝素蛋白的表位衍生出的标签肽。
靶点 anti-HA-tag antibody          
IC50            

质量控制

化学结构

Influenza Hemagglutinin (HA) Peptide

相关生物数据

Influenza Hemagglutinin (HA) Peptide

相关生物数据

Influenza Hemagglutinin (HA) Peptide

相关生物数据

Influenza Hemagglutinin (HA) Peptide

相关生物数据

Influenza Hemagglutinin (HA) Peptide

相关生物数据

Influenza Hemagglutinin (HA) Peptide