Hygromycin B
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hygromycin B是一种选择性抗生素，用于治疗和预防细菌感染。
Hygromycin B是一种抗生素，对大多数细菌、真菌和高等真核细胞有效。Hygromycin B具有30S亚基，含有单个结合位点，这与它的单相作用一致。该结合位点位于螺旋的大沟内，具有高度序列特异性。Hygromycin B通过抑制延长过程中的易位步骤和促使70S核糖体mRNA的错误翻译，抑制蛋白质合成[1]。在70S核糖体中，Hygromycin B选择性抑制核糖体ATP酶（RbbA）的活性，将RbbA从70S核糖体释放，RbbA增强16S rRNA开关螺旋区域A889和G890的反应性。另外，Hygromycin B可以保护解码区域的G1494和A1408。
参考文献：
[1]. Brodersen DE, Clemons WM Jr, Carter AP, et al. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell, 2000, 103(7): 1143-1154.
[2]. Ganoza MC, Kiel MC. A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob Agents Chemother, 2001, 45(10): 2813-2819.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 527.5 |
| Cas No. | 31282-04-9 |
| Formula | C20H37N3O13 |
| Solubility | ≥26.38 mg/mL in H2O; ≥5.35 mg/mL in EtOH; ≥9.14 mg/mL in DMSO |
| Chemical Name | (3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol |
| SDF | Download SDF |
| Canonical SMILES | CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Mouse L cells |
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Reaction Conditions |
200 μg/ml hygromycin B for 2 ~ 6 weeks |
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Applications |
Hygromycin B was used for resistance selection in mouse L cells transfected with Escherichia coli genes coding for resistance to the aminocyclitol antibiotics hygromycin B. |
| Animal experiment:[2] | |
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Animal models |
Four-week-old BALB/c mice susceptible to MHV-A59 infection |
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Dosage form |
0 ~ 10 μmol/kg Intraperitoneal (i.p.) injection |
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Applications |
A single injection of hygromycin B reduced the number of lesions in the livers of MHV-A59-inoculated mice. Higher doses of the drug appeared to be slightly more effective at reducing virus-induced lesions. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Santerre RF, Allen NE, Hobbs JN Jr, et al. Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. Gene, 1984, 30(1-3): 147-156. 2. Macintyre G, Curry B, Wong F, et al. Hygromycin B therapy of a murine coronaviral hepatitis. Antimicrobial Agents and Chemotherapy, 1991, 35(10): 2125-2127. |
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质量控制和MSDS
- 批次:
化学结构
