HPOB
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HPOB是高效的组蛋白去乙酰化酶6(HDAC6)选择性抑制剂,IC50值为56 nM,对于其他HDAC的有效性要弱30多倍[1].
HDAC6是十一种人类锌依赖HDAC之一,在C端拥有两个催化结构域和一个泛素结合域.HDAC6有多种生物学功能,包括调控微管动力学和降解错误折叠蛋白.HDAC6参与多个与正常和肿瘤细胞生长\迁移和死亡相关的细胞途径[1].
在正常HFS和改造的LNCaP细胞中,HPOB可抑制细胞生长而不抑制细胞活力,可诱导α-微管蛋白乙酰化,对组蛋白无功能.HPOB可在改造的细胞中提高依托泊苷\阿霉素和SAHA诱导的细胞死亡,在正常细胞中则不能.在改造的细胞中,HPOB可通过凋亡通路提高抗癌药物效果,并可激活DNA损伤应答[1].
HPOB在携带人类前列腺癌CWR22异种移植物的小鼠中耐受性良好,并可在这些动物中提高抗癌药SAHA的细胞毒性效应[1].
参考文献：
1. Lee JH, Mahendran A, Yao Y, Ngo L, Venta-Perez G, Choy ML, Kim N, Ham WS, Breslow R, Marks PA. Development of a histone deacetylase 6 inhibitor and its
biological effects. Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15704-9.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 314.34 |
| Cas No. | 1429651-50-2 |
| Formula | C17H18N2O4 |
| Solubility | ≥31.4 mg/mL in DMSO; insoluble in H2O; ≥11.4 mg/mL in EtOH |
| Chemical Name | N-hydroxy-4-(2-((2-hydroxyethyl)(phenyl)amino)-2-oxoethyl)benzamide |
| SDF | Download SDF |
| Canonical SMILES | OCCN(C(CC1=CC=C(C(NO)=O)C=C1)=O)C2=CC=CC=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
相关生物数据
