Hoechst 33342
Catalog No.
A3472
蓝色荧光染料
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Description:
IC50 Value: N/A
Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C.
The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.
Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.
in vitro: N/A
in vivo: N/A
Clinical trial: N/A
- 1. Shuling Wang, Xiaoyu Jiang, et al. "piR-823 inhibits cell apoptosis via modulating mitophagy by binding to PINK1 in colorectal cancer." Cell Death Dis. 2022 May 17;13(5):465. PMID: 35581181
- 2. Thipjutha Phatruengdet, Piyachat Khuemjun, et al. "Pharmacokinetic/Pharmacodynamic Determinations of Iron-tannic Molecular Nanoparticles with its Implication in MR Imaging and Enhancement of Liver Clearance." Nanotheranostics. 2022 Jan 1;6(2):195-204. PMID: 34976594
- 3. Yi Zhang, Dandan Zhang, et al. "Effects of RPE‑conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration." Exp Ther Med. 2017 Oct;14(4):3699-3707. PMID: 29042966
Storage | Store at -20°C |
M.Wt | 561.93 |
Cas No. | 875756-97-1 |
Formula | C27H31Cl3N6O |
Synonyms | bisBenzimide H 33342;HOE 33342;Hoechst-33342;Hoechst33342 |
Solubility | insoluble in EtOH; ≥28.7 mg/mL in H2O with gentle warming; ≥46 mg/mL in DMSO |
Chemical Name | 2'-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzo[d]imidazole trihydrochloride |
SDF | Download SDF |
Canonical SMILES | CCOC1=CC=C(C2=NC3=C(C=C(C4=NC5=C(C=C(N6CCN(C)CC6)C=C5)N4)C=C3)N2)C=C1.Cl.Cl.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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