HNHA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 100 nM
HNHA is an HDAC inhibitor.
Histone acetylation is regulated by two covalent modifying enzymes, acetylases and deacetylases, and plays a key role in gene expression of eukaryotes. It has been revealed that histone deacetylase (HDAC), which is overexpressed in several tumor cells. plays a critical role in carcinogenesis. Moreover, plenty of studies have showed that the expression of tumor suppressors including p53, p21, and gelsolin, are repressed, whereas, tumor activators including hypoxia-induced factor-1 and vascular endothelial growth factor, are up-regulated in HDAC-overexpressed cells.
In vitro: Previous study found that HNHA was able to inhibit in-vitro HDAC enzyme activity as well as proliferation of human fibrosarcoma cells (HT1080). In addition, treatment of cells with HNHA could elicite histone hyperacetylation resulting in an up-regulation of cell cycle arrest, p21 transcription, and an inhibition of HT1080 cell invasion [1].
In vivo: The effects of HNHA on human cancer cells bearing xenograph mice was examined. Results showed that as expected, HNHA could dramatically reduce tumor volume as to (vehicle) control. In addition, HNHA had a stronger potency than that of SAHA, which implied that the pharmacological potency of HNHA was better than SAHA in vivo [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
1. Kim, D.H.,Lee, J.,Kim, K.N., et al. Anti-tumor activity of N-hydroxy-7-(2-naphthylthio) heptanomide, a novel histone deacetylase inhibitor. Biochemical and Biophysical Research Communications 356, 233-238 (2007).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 303.4 |
Cas No. | 926908-04-5 |
Formula | C17H21NO2S |
Synonyms | Histone Deacetylase Inhibitor VI |
Solubility | ≤20mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide |
Chemical Name | N-hydroxy-7-(2-naphthalenylthio)-heptanamide |
SDF | Download SDF |
Canonical SMILES | c1ccc2c(c1)cc(cc2)SCCCCCCC(NO)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |