HJC 0350
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HJC 0350是EPAC2的有效选择性拮抗剂,IC50值为0.3 μM[1].
cAMP/cAMP调节鸟嘌呤核苷酸交换因子(EPAC/cAMP-GEF)是一种响应于细胞内cAMP的小GTP酶Rap1和Rap1的鸟嘌呤核苷酸交换因子.EPAC2主要表达于中枢神经系统\胰腺和肾上腺[1].
HJC 0350是EPAC2的有效选择性拮抗剂.HJC 0350与8-NBD-cAMP竞争结合重组融合蛋白EPAC2,IC50值为0.3 μM,是cAMP的133倍,而cAMP与8-NBD-cAMP竞争结合EPAC2,IC50值为40 μM.在25 μM cAMP存在下,25 μM的 HJC0350抑制EPAC2的GEF活性,但对EPAC1介导的Rap1-GDP的交换活性以及cAMP介导的PKA活化没有影响,这表明HJC 0350是EPAC2的特异性拮抗剂.在表达EPAC1或EPAC2荧光共振能量转移(FRET)传感器(EPAC2-FL或EPAC1-FL)的HEK293细胞中,10 μM的 HJC0350完全抑制HEK293/EPAC2-FL细胞中007-AM(膜可渗透的EPAC选择性cAMP类似物)诱导的FRET减少,但对HEK293/EPAC1-FL细胞没有影响[1].
参考文献:
[1]. Chen H, Tsalkova T, Chepurny OG, et al. Identification and characterization of small molecules as potent and specific EPAC2 antagonists. J Med Chem, 2013, 56(3): 952-962.
Storage | Store at -20°C |
M.Wt | 277.38 |
Cas No. | 885434-70-8 |
Formula | C15H19NO2S |
Solubility | insoluble in H2O; ≥10.84 mg/mL in EtOH with ultrasonic; ≥13.85 mg/mL in DMSO |
Chemical Name | 1-(mesitylsulfonyl)-2,4-dimethyl-1H-pyrrole |
SDF | Download SDF |
Canonical SMILES | O=S(N1C(C)=CC(C)=C1)(C2=C(C)C=C(C)C=C2C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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