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Hexa His tag peptide

现货
Catalog No.
A6006
合成的6×His肽
组合的产品项目
规格价格库存 数量
5mg
¥ 1,290.00
现货
25mg
¥ 5,180.00
现货

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Background

Hexa His is an amino acid sequence consisting of 6 His residues in a row, it is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Hexa His Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. Hexa His acts as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.

References:
1.  Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285–6.

文献引用

1. Chang M, Qin Q, et al. "Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein." J Sep Sci. 2018 Nov 28. PMID:30488556
2. Guo M, Tang X, et al. "Comparative study of the adjuvant potential of four Th0 cytokines of flounder (Paralichthys olivaceus) on an E. tarda subunit vaccine." Dev Comp Immunol. 2018 Sep;86:147-155. PMID:29746982
3. Bartoschik T, Galinec S, et al. "Near-native,site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis." Sci Rep. 2018 Mar 21;8(1):4977. PMID:29563556
4. Guo M, Tang X, et al. "The Immune Adjuvant Effects of Flounder (Paralichthys olivaceus) Interleukin-6 on E.tarda Subunit Vaccine OmpV." Int J Mol Sci. 2017 Jul 5;18(7). pii: E1445. PMID:28678171

Chemical Properties

StorageDesiccate at -20°C
M.Wt840.85
FormulaC36H44N18O7
SynonymsH-His-His-His-His-His-His-OH
Solubility≥84.1mg/mL in DMSO, ≥123.4 mg/mL in EtOH with ultrasonic, ≥67.5 mg/mL in H2O
Chemical NameHexa His
SDFDownload SDF
Canonical SMILESC1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

生物活性

描述 Hexa His tag peptide是6×His标签肽,用作重组蛋白的金属结合位点。
靶点 anti-6 x His tag antibody          
IC50            

质量控制

化学结构

Hexa His tag peptide

相关生物数据

Hexa His tag peptide

相关生物数据

Hexa His tag peptide

相关生物数据

Hexa His tag peptide