Hexa His tag peptide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hexa His是由6个组氨酸残基依次连接组成的氨基酸序列，也被称为hexa histidine-tag、6×His-tag或His-tag。Hexa His负责脑和其它组织中GM2神经节苷脂及各种末端包含N-乙酰己糖胺的降解。B形式对某些低聚糖是有活性的，而S形式没有可测量的活性。Hexa His作为重组蛋白的金属结合位点。His-Tag序列通过使用分子生物学公司提供的载体可以添加到靶蛋白的N末端或C末端。该标签后面连接的通常是特异性蛋白酶的酶切位点，如Leu-Val-Arg-Gly-Ser肽序列，被凝血酶识别并切割。
参考文献：
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285–6.
- 1. Guo M, Chen K, et al. "Bcl-2 mediates coelomocytes apoptosis by suppressing cytochrome c release in Vibrio splendidus challenged Apostichopus japonicus." Dev Comp Immunol. 2019 Oct 26:103533. PMID:31669879
- 2. Guo M, Lv M, et al. "Bax functions as coelomocyte apoptosis regulator in the sea cucumber Apostichopus japonicus." Dev Comp Immunol. 2019 Sep 5:103490. PMID:31494220
- 3. Chang M, Qin Q, et al. "Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein." J Sep Sci. 2018 Nov 28. PMID:30488556
- 4. Guo M, Tang X, et al. "Comparative study of the adjuvant potential of four Th0 cytokines of flounder (Paralichthys olivaceus) on an E. tarda subunit vaccine." Dev Comp Immunol. 2018 Sep;86:147-155. PMID:29746982
- 5. Bartoschik T, Galinec S, et al. "Near-native,site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis." Sci Rep. 2018 Mar 21;8(1):4977. PMID:29563556
- 6. Guo M, Tang X, et al. "The Immune Adjuvant Effects of Flounder (Paralichthys olivaceus) Interleukin-6 on E.tarda Subunit Vaccine OmpV." Int J Mol Sci. 2017 Jul 5;18(7). pii: E1445. PMID:28678171
| Storage | Desiccate at -20°C |
| M.Wt | 840.85 |
| Formula | C36H44N18O7 |
| Synonyms | H-His-His-His-His-His-His-OH |
| Solubility | ≥84.1 mg/mL in DMSO; ≥123.4 mg/mL in EtOH with ultrasonic; ≥67.5 mg/mL in H2O |
| Chemical Name | Hexa His |
| SDF | Download SDF |
| Canonical SMILES | C1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | Hexa His tag peptide是6×His标签肽，用作重组蛋白的金属结合位点。 | |||||
| 靶点 | anti-6 x His tag antibody | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
相关生物数据
相关生物数据
