Heparin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
肝素是高度硫酸化的糖胺聚糖,广泛用作可注射的抗凝血剂。肝素仅存在于动物界的少数组织和物种中,在特殊细胞的细胞质中的细胞器内的以颗粒形式存在。肝素可能参与针对细菌和其他外来物质的防御机制[1]。
体外实验:肝素(25-500 Nμ/ ml)以剂量依赖性方式抑制由FMLP或通过酵母聚糖活化的血清诱导的细胞聚集和脱颗粒。25 μg/ml的肝素特异性抑制FMLP依赖超氧化物阴离子产生和化学发光。肝素抑制功能和代谢粒细胞激活的所有方面[2]。
在体实验:在来自肝素处理大鼠的细胞或肝素处理的细胞中,由于血管紧张素II受体的数量和亲和力降低,[125I]碘-血管紧张素II的特异性结合降低。在肝素处理大鼠的细胞和肝素处理的细胞中,肝素还降低了血管紧张素II诱导的醛固酮产生的最大值。肝素与肾上腺血管紧张素II受体相互作用从而抑制血管紧张素II诱导的醛固酮产生[3]。
参考文献:
[1]. Nader H B, Chavante S F, Dos-Santos E A, et al. Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates[J]. Brazilian Journal of Medical and Biological Research, 1999, 32(5): 529-538.
[2]. Pasini F L, Pasqui A L, Ceccatelli L, et al. Heparin inhibition of polymorphonuclear leukocyte activation in vitro. A possible pharmacological approach to granulocyte-mediated vascular damage[J]. Thrombosis research, 1984, 35(5): 527-537.
[3]. Azukizawa S, Iwasaki I, Kigoshi T, et al. Effects of heparin treatments in vivo and in vitro on adrenal angiotensin II receptors and angiotensin II-induced aldosterone production in rats[J]. Acta endocrinologica, 1988, 119(3): 367-372.
Storage | Store at -20°C |
M.Wt | 1134.93 |
Cas No. | 9005-49-6 |
Formula | C26H42N2O37S5 |
Solubility | ≥12.75mg/ml in H2O |
SDF | Download SDF |
Canonical SMILES | CC(NC1C(O)C(OC2C(OS(=O)(O)=O)C(O)C(OC3C(NS(=O)(O)=O)C(OS(=O)(O)=O)C(OC4C(OS(=O)(O)=O)C(O)C(O)C(C(O)=O)O4)C(CO)O3)C(C(O)=O)O2)C(COS(=O)(O)=O)OC1O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |