Gedunin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Gedunin是一种天然发生的Hsp90抑制剂,是一种从印度楝树(Azadirachta indica)中分离的tetranortriterpenoid。
Hsp90(热休克蛋白90)是一种稳定蛋白质免于热应激的伴侣蛋白,帮助蛋白降解。最初该蛋白从热、脱水或其他手段应激下的细胞中抽提蛋白时得到。该物质也可稳定多种肿瘤生长必需的蛋白,因此Hsp90抑制剂可被研发为抗癌药。
在体外,Gedunin可诱导Hsp90依赖的客户蛋白降解,并在SKBr3、CaCo-2和MCF-7癌细胞系中具有抗增殖活性,IC50值分别为3.22、16.8和8.84 μM[1]。
在寄生虫发育和[3H]-次黄嘌呤摄入试验中,Gedunin治疗具有针对恶性疟原虫的抗疟疾活性,IC50值分别为0.14和3.1 μM[2]。在小鼠模型中,gedunin对酵母多糖诱导的发炎膝关节具有显著的抗炎和止痛效果,以及不同细胞群的调节[3]。
参考文献:
1. Uddin SJ, Nahar L, Shilpi JA, et al. Gedunin, a limonoid from Xylocarpus granatum, inhibits the growth of CaCo-2 colon cancer cell line in vitro. Phytotherapy research: PTR. 2007;21(8):757-761.
2. Lee SE, Kim MR, Kim JH, et al. Antimalarial activity of anthothecol derived from Khaya anthotheca (Meliaceae). Phytomedicine: international journal of phytotherapy and phytopharmacology. 2008; 15(6-7):533-535.
3. Conte FP, Ferraris FK, Costa TE, et al. Effect of gedunin on acute articular inflammation and hypernociception in mice. Molecules. 2015; 20(2):2636-2657.
Physical Appearance | White solid |
Storage | Store at -20°C |
M.Wt | 482.57 |
Cas No. | 2753-30-2 |
Formula | C28H34O7 |
Solubility | Soluble in DMSO |
Chemical Name | (1R,3aS,4aR,4bR,5S,6aS,10aR,10bS,12aS)-1-(furan-3-yl)-4b,7,7,10a,12a-pentamethyl-3,8-dioxo-1,3,3a,4b,5,6,6a,7,8,10a,10b,11,12,12a-tetradecahydronaphtho[2,1-f]oxireno[2,3-d]isochromen-5-yl acetate |
SDF | Download SDF |
Canonical SMILES | O=C1O[C@H](C2=COC=C2)[C@@]3(C)[C@]4([C@]5(C)[C@@H](CC3)[C@@](C)(C=C6)[C@@H](C(C)(C)C6=O)C[C@@H]5OC(C)=O)O[C@@H]41 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |