Fosmidomycin (sodium salt)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Fosmidomycin is a specific inhibitor of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXP) [1]. Fosmidomycin is an antibiotic originally isolated from bacteria of the genus Streptomyces.
1-deoxy-d-xylulose 5-phosphate reductoisomerase is an enzyme that interconverts DXP and 2-C-methyl-D-erythritol 4-phosphate (MEP) [2].
Fosmidomycin was active against both Gram-negative and Gram-positive bacteria and the human malarial parasite P. falciparum with the IC50 of 290-370 nM[3]. Fosmidomycin inhibited 1-deoxy-d-xylulose 5-phosphate reductoisomerase in an alternative nonmevalonate pathway for terpenoid biosynthesis with IC50 of 8.2 nM [1]. Fosmidomycin has been shown to possess activity against Plasmodium falciparum in vitro and in the mouse model. In patients with acute uncomplicated Plasmodium falciparum malaria, oral administration of 1,200 mg fosmidomycin every 8 h for 7 days was effective in curing uncomplicated Plasmodiumfalciparum malaria in humans [4]. Fosmidomycin was an effective and safe antimalarial drug [4]. The treatment was well tolerated and showed a fast parasite and fever clearance [4].
References:
[1] Kuzuyama T, Shimizu T, Takahashi S, et al. Fosmidomycin, a specific inhibitor of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in the nonmevalonate pathway for terpenoid biosynthesis[J]. Tetrahedron letters, 1998, 39(43): 7913-7916.
[2] Takahashi S, Kuzuyama T, Watanabe H, et al. A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis[J]. Proceedings of the National Academy of Sciences, 1998, 95(17): 9879-9884.
[3] Jomaa H, Wiesner J, Sanderbrand S, et al. Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs[J]. Science, 1999, 285(5433): 1573-1576.
[4] Lell B, Ruangweerayut R, Wiesner J, et al. Fosmidomycin, a novel chemotherapeutic agent for malaria[J]. Antimicrobial agents and chemotherapy, 2003, 47(2): 735-738.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 205.1 |
Cas No. | 66508-37-0 |
Formula | C4H9NO5P·Na |
Synonyms | Antibiotic FR31564,FR31564 |
Solubility | ≤5mg/ml in PBS, pH 7.2 |
Chemical Name | P-[3-(formylhydroxyamino)propyl]-phosphonic acid, monosodium salt |
SDF | Download SDF |
Canonical SMILES | ON(C([H])=O)CCCP([O-])(O)=O.[Na+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
恶性疟原虫菌株 |
溶解方法 |
在PBS中的溶解度≤5mg/ml,pH 7.2。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
45nM~370nM |
应用 |
Fosmidomycin对三种不同的恶疟原虫菌株(HB3、A2和Dd2)均有活性,IC50值为45nM~370nM。 |
动物实验 [1]: | |
动物模型 |
啮齿动物温氏疟原虫感染小鼠 |
剂量 |
10 mg/kg, 腹腔注射 |
应用 |
用大于10 mg / kg的fosmidomycin腹腔注射处理,动物显然不含寄生虫。用5 mg / kg的fosmidomycin处理,寄生虫血症 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: [1]. Jomaa H, Wiesner J, Sanderbrand S, Altincicek B, Weidemeyer C, Hintz M, Türbachova I, Eberl M, Zeidler J, Lichtenthaler HK, Soldati D, Beck E. Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs. Science. 1999 Sep 3;285(5433):1573-6. PubMed PMID: 10477522. |