Flt3-Ligand, human recombinant
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Human FMS-like tyrosine kinase 3 ligand (Flt3-ligand) is a cytokine that synergizes with other CSFs and interleukins to induce proliferation and differentiation of early hematopoietic cells. Human Flt3-ligand cDNA encodes a 235 amino acid residue precursor protein that is cleaved to yield mature human Flt-3 ligand, which consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Recombinant human flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues, which comprises the extracellular domain of the transmembrane flt3-ligand protein.
| Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. | ||||||
| ED50 | < 0.1 ng/ml. | |||||
| Specific activity | > 1*106 units/mg. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
相关生物数据
| Gene ID: | 2323 | Accession #: | P49771 |
| Alternate Names: | Flt3-L, human flt3-Ligand, flt3-Ligand, flt3Ligand, flt3 Ligand,h-flt3-Ligand, rh-flt3-Ligand, recombinant human flt3-Ligand, recombinant flt3-Ligand. | ||
| Source: | Escherichia Coli. | M.Wt: | 17.6 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized with no additives. The protein may appear as a haze of film, which is difficult to see at the bottom of the vial. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||